Replication & Transcription

Replication One of the hallmarks of living organisms is their ability to reproduce. DNA contains the genetic information

The interrelationship of DNA,RNA & Protein

DNA Contains Four Deoxynucleotides

Double-stranded DNA

Formation of hydrogen bonds between complementary bases in double-stranded DNA

Base pairing

Supercoiling of DNA. DNA Exists in Relaxed & Supercoiled Forms

Negative and positive supercoils.

Important structural elements of a yeast chromosome

extent of DNA packaging in metaphase chromosomes

Requirements for DNA Replication

DNA provides a template for Replication & transcription

DNA replication is semiconservative

Steps involved in DNA replication in eukaryotes

Classes of proteins involved in replication

The initiation of DNA synthesis upon a primer of RNA

DNA polymerase III

A comparison of prokaryotic and eukaryotic DNA polymerases

DNA Polymerases

The telomere replicacion problem

Cyclins and cyclin-dependent kinases involved in cell cycle progression

Cell cycle

RNA Synthesis, Processing, & Modification

Prokaryotic gene PromoterCoding regionTermination

Introns in two eukaryotic genes.

Promoter structure

Bacterial promoters

Eukaryotic Promoters Are More Complex

the transcription control regions

Summary of the properties of enhancers.

Nomenclature and properties of mammalian nuclear DNA-dependent RNA polymerases.

Structure of a typical eukaryotic mRNA showing elements that are involved in regulating mRNA stability

Typical aminoacyl tRNA

Classes of eukaryotic RNA.

Components of mammalian ribosomes