H. M.J.Bandara Plant Quarantine Officer Sri Lanka Biotechnology: Presant Status & biosafety concerns Department of Agriculture H. M.J.Bandara Plant Quarantine Officer Sri Lanka

Department of Agriculture Mandatory Crops: Cereals, legumes, condiments, fruit crops, root & tuber crops, oil seed crops vegetables, floricultural plants Activities: Research & development, Seed certification & plant protection, Planting material production Distribution & Plant quarantine, Germplasm management, Botanic gardens, Agriculture extension, Education & training, Finance & administration

Tissue Culture Molecular techniques (Based on nuclear acid & proteins) Biotechnology Tissue Culture Molecular techniques (Based on nuclear acid & proteins) Biotechnology is defined in many ways. Tissue culture is considered one of the techniques. It’s associated directly with genetic transformation.

Banana Tissue Culture Papaya tissue culture HORTICULTURAL CROP RESEARCH AND DEVELOPMENT INSTITUTE- Tissue Culture Program Banana Tissue Culture Papaya tissue culture

HORTICULTURE CROP RESEARCH AND DVELOPMENT INSTITUTE Virus indexing (serological) Banana Papaya Citrus Microbiology Food Research Unit Fermentation Food analysis

PLANT VIRUS INDEXING CENTER Serology and PCR Based Virus Indexing- Banana Virus Pineapple RT PCR for Detection of RNA Virus

SITHA ELIYA POTATO RESEARCH CENTER: Tissue Culture Program Potato Straw berry Pineapple

RARDC, Bandarawela Tissue Culture Program Potato Anthurium Gerbera Strawberry Madonna lily Virus indexing of potato by ELISA

RARDC, Agunakolapelessa Banana Thubakarwial

RICE RESEARCH AND DEVELOPMENT INSTITUTE:Rice Biotechnology Anther Culture Exploitation of Somaclonal Variation Wide Hybridization

NATIONAL BOTANIC GARDENS-Tissue Culture Program Propergation of Endemic & rare Plants Floriculture Plants Mutation induction

PLANT GENETIC RESOURCE CENTER: Tissue Culture Program Banana Citrus spp In vitro pineapple potato Conservation Papaya tissue Culture

PLANT GENETIC RESOURCE CENTER: Tissue Culture Program Anther Culture in rice Haploid Culture a good system for transformation

PGR/RRDI- Rice research PCR based DNA markers for breeding/gene pyramiding Gall midge resistance Thrips resistance Bactria leaf blight resistance BPH resistance

Genetic Characterization by DNA markers Mango - Mangifera zeylanica Magifera indica Maize(PGRC/MI) Finger printing of hybrids Pathogens - Ralstonia, BLB Onion - Characterization & breeding

Introgression of genes from wild relatives Wild tomato X tomato Wild okra X okra

Future Work SSR markers for rice AFLP for fingerprinting of germplasm & Varieties Northern blot techniques for Analysis of HSP regular gene Expression Isolating the fragment for Coat protection gene papaya Ring spot virus

Application of Tools in Biosafety Regular Measures Tissue Culture Safe handling of materials Restricted multiplication Serological methods Testing &detection of foreign Genes bases in proteins Serological method should not be the sole method. Genes expression (transgenic)is spatially & temporally controlled PCR/Blot methods Based on DNA More sensitive method.Detect only presence or foreign DNA

National Plant Quarantine Service Requirements With the existing infrastructure & function and provided with additional strength (Trained staff, Detection equipment, Risk assessments Studuo) NPQS could undertake the regulatory activity

Institutional Biosafety Committee Liaison with the national committees Identification of institutes:transformation work,GMO detection /testing,regulation & risk assessment Preparation of guidelines on biosafety regulations,risk assessments & management Maintain constant access to relevant data base or registers both local & foreign Monitoring & evaluation of programs Building awareness

Requirements for Implementation of the Biosafety Procedures Policy level support Financial,administrative & legislative support Biosafety clearing house mechanism Monitoring, evaluation & inspection services Training of decision makers,&technical level scientists & technicians Facility enhancement Public awareness, education & promotion of public participation including stake holders.

GENETIC Transformation of Agricultural Crops Policy level decisions & if necessary, changes Identification of priority problems/crops Identification of Institutes Facility development

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