Medical Enzymology By Amr S. Moustafa, M.D.; Ph.D. Assistant Prof. & Consultant, Medical Biochemistry Dept. College of Medicine, KSU

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Presentation transcript:

Medical Enzymology By Amr S. Moustafa, M.D.; Ph.D. Assistant Prof. & Consultant, Medical Biochemistry Dept. College of Medicine, KSU

Enzymes: Recommended Names  Substrate + -ase e.g., Sucrase, Lactase  Action: Lactate dehydrogenase  Others: Pepsin, Trypsin

Enzymes: Systematic Names  Lactate:NAD + Oxidoreductase  Complete description of chemical reaction  Six major classes of enzymes

Enzymes: 6 Major Classes

Aminotransferases

Hydrolases

Isomerases

Ligases

Substrate Concentration: Hyperbolic Vs Sigmoid Curve

Michaelis-Menten Equation  E + S ES E + P  v ° = K m : Michaelis constant = (k-1 + k2) / k1 k1 K-1 k2 V max [S] K m + [S]

Michaelis-Menten Kinetics

Zero and First Order Reactions

Lineweaver-Burk Plot = + 1 v°v° KmKm V max [S] 1 V max