1. Enzyme Catalysis SBS017 Basic Biochemistry Dr John Puddefoot J.R.Puddefoot@qmul.ac.uk

2. Learning objectives Lecture 11 You should be able to use Lineweaver-Burk plots to calculate KM and Vmax You will be able to describe competitive, uncompetitive and non-competitive inhibitors and how they alter enzyme kinetics You should understand the terms Allosteric and Cooperative regulation in relation to enzymes

3. Michaelis-Menten Reminder V is the velocity (or rate) of reaction [S] is the substrate concentration Vmax is the maximal velocity of the reaction K M is the Michaelis constant ([S] at Vmax/2)

5. K +1 [E][S] = k -1 + k 2 [ES]

23. App