MRMPilot ™ Software © 2008 Applera Corporation and MDS Inc.

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Presentation transcript:

MRMPilot ™ Software © 2008 Applera Corporation and MDS Inc.

MRMPilot ™ Software © 2008 Applera Corporation and MDS Inc. AB SCIEX Protein Research Solutions for Targeted Quantitative Proteomics Create robust peptide MRM assays without protein standards Choose the best MRM using the MIDAS ™ workflow Import proteins directly from discovery data Built-in fragmentation rules for PTM’s, mTRAQ ™ reagents, and more

MRMPilot ™ Software © 2008 Applera Corporation and MDS Inc. Q: But how do you create a robust MRM for Targeted Proteomics without the use of peptide standards? MRM assays offer the highest selectivity and sensitivity for quantitatively detecting peptides… A: By combining the unique MIDAS ™ Workflow of the QTRAP ® System and the automated MRMPilot ™ software. AB SCIEX Protein Research Solutions for Targeted Quantitative Proteomics One touch productivity.

MRMPilot ™ Software © 2008 Applera Corporation and MDS Inc. Data from discovery experiments can be imported manually or automatically into MRMPilot ™ software… AB SCIEX Protein Research Solutions for Targeted Quantitative Proteomics

MRMPilot ™ Software © 2008 Applera Corporation and MDS Inc. The method is run by LC MRM on the biological sample of interest for MRM optimization. AB SCIEX Protein Research Solutions for Targeted Quantitative Proteomics The best theoretical MRM transitions can be chosen and the method built.

MRMPilot ™ Software © 2008 Applera Corporation and MDS Inc. The unique MIDAS ™ workflow can be enabled to obtain high-sensitivity, full-scan MS/MS for peptide confirmation with a simple tick in a box. AB SCIEX Protein Research Solutions for Targeted Quantitative Proteomics

MRMPilot ™ Software © 2008 Applera Corporation and MDS Inc. Full scan MS/MS from the MIDAS ™ workflow provides peptide ID confirmation and can be used to identify interferences… One touch productivity. The optimized, robust MRM assays can now be used with confidence to quantify peptides and proteins in hundreds of biological samples. AB SCIEX Protein Research Solutions for Targeted Quantitative Proteomics Only MRM labeled as “Optimized” will be included in the final assay. The MRM table makes choosing the most sensitive MRM transition for detecting the protein easy. The actual MRM data are imported back into MRMPilot ™ Software for analysis and optimization.