BACTERIALBASICS.

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Presentation transcript:

BACTERIALBASICS

Bacteria Structure Prokaryotes Use asexual reproduction No nucleus Cell wall DNA plasmid (ring-shaped) Use asexual reproduction http://en.wikipedia.org/wiki/Bacteria

Different Forms of DNA Transfer

Bacterial Transformation 1928-Frederick Griffith, 1944-Avery, McCarty, Macleod Process of taking up DNA from media DNA can be from cells from same or different species DNA will integrate into the recipient’s chromosome http://en.wikipedia.org/wiki/Bacterial_transformation

Bacterial Adhesion Zones Adhesion zones develop in the bacteria cell membrane, although DNA cannot fit through

Calcium Chloride Solution The addition of calcium ions in CaCl2 solution shields the negatively charged phosphate groups so that the DNA can pass through the membrane when heat shocked. Heat Shock 42ºC