2004-2005 Lab 6: Molecular Biology Description – Gel electrophoresis cut DNA with restriction enzyme fragments separate on gel based on size.

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Presentation transcript:

Lab 6: Molecular Biology Description – Gel electrophoresis cut DNA with restriction enzyme fragments separate on gel based on size

Lab 6: Molecular Biology – INTRODUCTION: – restriction enzyme – gel electrophoresis DNA is negatively charged smaller fragments travel faster RFLPs DNA Fingerprint

Hypothesis IND VAR: DNA samples DEP VAR: RFLP fragment sizes PREDICT: who is the criminal?

Lab 6: Gel Electrophoresis Data and analysis DNA = negatively charged smaller fragments travel faster & therefore farther correlate distance to size

DATA Sketch of gel Data table from pg. 43 Logarithmic graph.

Analysis Answer “interpretation of results” on p. 46

Questions Answer all questions asked DURING THE LAB at end of lab. Pp. 29, 34, 36

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