Prostate: Transverse section

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Presentation transcript:

Prostate: Transverse section

Radical Prostatectomy Bladder neck Apex

Radical Prostatectomy Base/ Bladder Neck Apex Posterior quadrants of alternate slices have been removed and frozen after inking the surface

Fresh, non-frozen tissue Lateral aspect Schematic of how samples of tumor are obtained from a radical prostatectomy specimen, without compromising sampling or diagnostic pathology data Seminal Vesicle Permanent section Permanent section right left 1 2 6 7 3 4 5 5R 5 5R 5L 5L Fresh, non-frozen tissue for flow sorting Frozen section Unfixed tissue for RNA & DNA right left 4R Frozen section 4 4R 4L 4L Permanent section right left permanent section 3R 3 3R 5L 3L = Prostate carcinoma

Fresh, non-frozen tissue: cell sorting Human prostate (lateral view) Obtaining samples of non-tumor tissue from a radical prostatectomy specimen. Seminal Vesicle Permanent section Fixed tissue: In-situ hybridization 1 2 6 7 right left 3 4 5 5R 5 5R 5L 5L Fresh, non-frozen tissue: cell sorting This block is snap frozen (isopentane in liquid N2), within < 20 minutes of devitalization Frozen tissue: Immunostains Laser capture right left 4R 4 4R 4L 4L Frozen section Permanent section right left Permanent section 3R 3 3R 5L 3L = Prostate carcinoma

Prostate (transverse section)

Normal prostate parenchyma (high magnification) Basal epithelial cells Secretory (luminal) epithelial cells Fibromuscular stromal cells

Normal prostate parenchyma (low magnification) fibromuscular stroma normal glands lymphocytes