Bacterial culture in laboratory

Slides:

Advertisements

Similar presentations

Lab # 3 Aseptic Transfer Techniques

Advertisements

ASEPTIC TECHNIQUE. PERSONAL PROTECTIVE EQUIPMENT includes: Eye protection Gloves Masks Gowns Sterile technique is employed to prevent contamination of.

Aseptic Technique: Media and Equipment

Bacterial Generation Time

Sterilization and disinfection. Sterilization can be define as the process by which all forms of microbial life including bacterial spores & vegetative.

Lab procedures when handling micro-organisms Distribution of micro-organisms in nature.

TEKS 2(E) plan and implement (carry out) investigative procedures, including asking questions, formulating testable hypotheses, and selecting equipment.

The Serial Dilution Method of Bacteria Enumeration

Isolation technique with aseptic techniques to cultivate bacteria

PHT 381 Lab# 4. A Culture medium:- ❊ An artificial preparation which contains the essential elements and nutrients needed by the m.o to grow. (most.

ASEPTIC TECHNIQUE Removing inoculum from a broth culture

ASEPTIC TECHNIQUE Removing inoculum from a broth culture

PHT 381 Lab# 4. A Culture medium:- ❊ An artificial preparation which contains the essential elements and nutrients needed by the m.o to grow. (most.

Introduction to Lab Ex. 19: Enumeration of Bacteria

Culture Media Lab 2:.

Determination of the origin of the contamination of the patient.

G ROWING BACTERIA. The action of antibiotics and disinfectants can be investigated using cultures of microorganisms (populations of microorganisms that.

Laboratory Equipment Biology.

How to inoculate culture media

Aseptic Techniques Noadswood Science, 2012.

Dalia kamal Eldien Mohammed. Material:  Sensitive balance  Flask  Measuring cylinder  Filter paper  Distilled water  Bunsen burner  Autoclave 

Aseptic Technique: Media and Equipment. Growth Medium A growth medium or culture medium is a liquid or gel designed to support the growth of microorganisms.

2A: Microscopy. Post Lab 2 is assigned today and due by the time your lab meets next. Pre Lab 3 will be available on Wednesday at 5 PM and is also due.

Biosafety.  NO FOOD OR DRINKS!  Wash hands thoroughly  Disinfect counters and work area  Tie hair back  Smock, apron, or lab coat optional  Gloves.

Culturing Yeast Cells on Media. Pre Lab Definitions: Petri Dish: A round, shallow dish used to grow bacteria. Culture: To grow living organisms in a prepared.

Ex. 7: Aseptic Technique – Inoculating Different Types of Media Key concepts: 1.Different types of bacterial cultures: broth, agar slant, and agar deep.

Bacterial culture Introduction. What are bacteria?  Prokaryotic organism  Unicellular  No nuclear envelope around the genomic DNA  Absence of cellular.

Lab 2: Culture Media. In this lab we learn about different types of media that are used to grow bacteria. Some types of media will grow just about any.

General Microbiology Laboratory Sterile Technique & Pure Culture Concept.

LAB 4: ASEPTIC TECHNIQUE AND ISOLATION OF BACTERIA

Supplemental instruction Chattahoochee Technical College

Practical Part Microscopic Examination of Microorganisms Experiments Identification of MOs Different Staining Techniques.

Lab safety rules practical NO (1) Dalia Kamal Eldien Mohammed.

Microbiology / Lab. 8. o Culture (Growth) Media I.What is a medium (plural media)? II.What is culture medium? III.What is meant by Inoculation of Media?

Petri Dish Used to grow cultures of bacteria or fungi.

General Microbiology Laboratory Bacterial Generation Time.

1. 2 Microbiology is the science that deals with the study of too small organism (micro-organisms) that are invisible to the naked eye.

Pure Culture Techniques

Introduction In natural environments, microorganisms usually exist as mixed populations. However, if we are to study, characterize, and identify microorganisms,

Antibiogram realisation on a bacterial strain. Materials avalaible Swab Agar with bacteria Antibiotics disks Mueller Hinton culture medium Deposit plan.

Bacterial identification plating streaking how to inoculate how to observe.

Lab Exercise 2 Culture Media. General Purpose Media: Nutrient Broth and Nutrient Agar.

1 Dr. Paul KONKUK UNIVERSITY DEPT. OF ENVIRONMENTAL ENGINEERING INTRODUCTION TO MICROBIOLOGY LABORATORY.

Aerosols Sneeze simulation method. Aerosols Suspension of a microbe containing particle Airborne pathogenic microorganisms Constitute a major hazard to.

Microbial Biotechnology Reem Alsharief Lab 3. General Methods of Isolation and selection of Microorganism Microbial isolation: To separate (a pure strain)

Isolation of microorganisms

Bacterial Cultures *Bacteria grow best in warm, moist, dark areas that contain a lot of food. -When we culture bacteria, we provide them with this environment.

ASEPTIC TECHNIQUE.  Laboratory practices that minimize the risk of cultured cells becoming contaminated with unwanted microorganisms.

Microbiological Tools To assist with transferring cultures to the different types of media there are a few different types of tools that can be utilized.

Basic Microbiology and Immunology Practical Course 2016.

Inoculation Incubation Isolation Inspection Identification 5/6/2018

Sterile Technique & Pure Culture Concept

Lab procedures when handling micro-organisms

Marine Biotechnology Lab

Aim: To prepare pure culture by streak plate method.

Learning Objectives i Understand the basic aseptic techniques used in culturing organisms. ii Understand the principles and techniques involved in culturing.

Bacterial Transformation

Culture Media Lab 2:.

Lab procedures when handling micro-organisms

Bacterial Transformation and GFP stories

General Microbiology Sterilization 140 MIC Lab 1 :

Antibiotic sensitivity test

Aseptic Technique & Streaking for Isolation

Handling of Bacterial Cultures

FOOD SCIENCE LAB EQUIPMENT

STATION 1 STATION 1 SAFETY SAFETY.

Bacterial degradation of several plastics:

Presentation transcript:

Bacterial culture in laboratory

Bacterial culture in laboratory Introduction : bacterial growth

Bacterial growth Multiplication by binary fission: the bacteria grows and divides into two identical daughter cells. Characterized by a generation time (G) variable depending on the species 20 minutes for Escherichia coli, Lactobacillus acidophilus for 100 minutes, 1000 minutes to Mycobacterium tuberculosis

Bacterial growth

Bacterial growth After n cycles : 2n bactéria

Bacterial growth For example, Escherichia coli G= 20 minutes After 1 hour: 3 cycles : n = 23 = 8 bacteria After 2 hours: 6 cycles : n = 26 = 64 bacteria After 3 hours : 9 cycles : n = 29 = 512 bacteria After 4 hours : 12 cycles: n = 212 = 4096 bacteria After 5 hours : 15 cycles: n = 215 = 32768 bacteria … After 10 hours : 30 cycles : n = 230 = 1073741824 bacteria More than a billion bacteria after 10 hours of culture!

Bacterial culture in laboratory Culture media and inoculation technique

Culture media Solid by adding a gelling agent (agar) to a liquid media

Culture in liquid media After 16h of culture = turbid media Bacteria Increasing the cell density during the growth Clear media

1 bacteria  1 visible bacterial colony Culture in solid media Bacteria After 16 - 24h : 1 bacteria  1 visible bacterial colony Accumulation of cells during the growth

Inoculation technique In liquid media : In solid media (usually with Agar Petri dish) : Inoculation with pipette Spreading with a rake Ridges with a loop Inoculation with loop Ridges with a swab

Bacterial culture in laboratory Aseptic conditions in lab

Aseptic conditions in lab Problem 2 : Bacteria can contaminate the technician Problem 1 : Bacteria from environment can contaminate the preparation Bacteria to study… Problem 3 : Technician can contaminate the preparation Need a sterile zone around the preparation !!

Aseptic conditions in lab Equipment to create a sterile area to work… Microbiological Safety Cabinet : System which uses an HEPA filter (High Efficiency Particulate Air) to generate a flow of sterile air  Protection for the preparation and the technician

Aseptic conditions in lab Equipment to create a sterile area to work… Bunsen Burner : Gas burner which delivers a flame at 1300°C  provides a sterile air zone near

Aseptic conditions in lab Working with a Bunsen Burner : Sterile air area : Approximately 40 cm diameter around the burner This area is use to open tube or agar Petri dish with bacteria Convection air movement

Aseptic conditions in lab Organization of the workspace Bunsen Burner is place in the center of the workspace and all instruments are distributed around (aerial view) : Rack tubes Bunsen Burner Box with instruments (pipettes, inoculation loops…) Trash for contaminated instruments Agar Petri dish

Aseptic conditions in lab Organization of the workspace

Aseptic conditions in lab Consumables materials Most microbiology labs use sterile disposable equipment : Inoculation needles and loops Volumetric pipettes Spreading rake Pipettes