Impact of INBRE Program at the University of Arkansas at Pine Bluff

Progress Report Funding Sources Biomedical & Behavioral Research Grants # of Applications Submitted Number of Awards Total $ All sources – total Faculty ? 6 $825,125 NIH sources – All Faculty 1 1 $25,000 NIH Sources – INBRE Faculty 1 1 $25,000

Progress Report Science & Health Related Degrees Associates Bachelors Masters Doctorate Degrees being pursued Degrees Awarded Yes No Faculty Release time X Central Grants Administration X

Progress Report Faculty Served on Peer Review Groups Number Within this institution Outside this institution 3 4 Total Research Space 17,320 square feet

Progress Report Total on Roster this reporting period 2 Independent Status Achieved 0 with Research or Program Project Grant (b) 0 without Research or Program Project Grant 0 No Longer Participating 0 Still Junior Investigator at Reporting Period End 2

Project #1 Assessment of Drug Uptake and Permeability Properties of Novel Anticancer Agents across Blood-brain barrier Dr. Antonie Rice

Rice Laboratory

Rice Project – Student Progress Students Year Total hours spent in the lab Major Expected grad. date Johnmesha Sanders 2nd Year Masters 25-30/wk Chemistry Education May 2010 Shanika Smith Senior 25-30/wk Chemistry Dec Phillisia Sims Sophomore 20-25/wk Chemistry May 2012 Trenton Ware Sophomore 20-25/wk Chemistry May, 2012 Students working in your lab (previous and present) applying/accepted to graduate school: Student Institutions applying to Area of study Ryan Watley Accepted to Univ. Of Oklahoma PhD. Program in Chemistry Shanika Smith Purdue and University of Kansas Medicinal Chemistry Johnmesha Sanders UAPB (accepted) UAMS (applying) Chem. Education (MS)

Rice Project - Publications “Characterization of the OAT, MCT and NaDC Transporters in Bovine Brain Microvessel Endothelial Cells (BMEC)” (submitted to J. of Mol Neuroscience) “Determination of Mechanistic Pathway of Chemically Modified Taxane with Enhanced Permeation across the Blood–Brain Barrier” (submitted to J. of Pharmaceutical Research)

Rice Project - Presentations National Presentations *Rice, A.H, Sanders, Johnmesha, Smith, Shanika “Characterization of Active Transporter Systems at Blood- Brain Barrier” NOBCChE Conference, St. Louis, MO April *Rice, A.H, Sanders, Johnmesha, Smith, Shanika “Characterization of Active Transporter Systems at Blood- Brain Barrier” National AAPS Conference, Atlanta,Georgia November Local Presentations *Rice, A. H., Sanders, Johnmesha, Audus, K.L., Georg, G., “Characterization of NaDC Transporter and P- glycoprotein Substrates in Bovine Brain Microvessel Endothelial Cells” UAPB Annual Research Symposium, Pine Bluff, AR, March Regional Presentations *Rice, A.H., Smith, Shanika, Sanders, Johnmesha, “Characterization of Active Transporters at Blood-Brain Barrier” ARK-LSAMP Regional Conference, Little Rock, AR, April National Presentations *Rice, A.H., Smith, Shanika, Sanders, Johnmesha, “Characterization of Active Transporters OAT and NaDC at Blood-Brain Barrier” National HBCU-UP Conference, Washington, D.C. October 2009.

Rice Project – Equipment Used Biotek 96-well Fluorescent Plate reader $22,000 INBRE Thermo Fisher Nicolet x700 FTIR Spectrometer $30,000 Title III Shimadzu HPLC $50,000 Title III

Project #2 Metal Chelates in Prevention of Cisplatin- Induced Kidney Cell Death Dr. Grant Wangila

Wangila Laboratory

Wangila Project: Student Progress Student Year hours Major expected grad. date Green, Ashlee Senior 720 Chemistry Graduated Dec 2009 Charvon Cade Senior 800 Chemistry May 2010 Brian Holmes Soph. 120 Chemistry May 2012 Lillian Johnson Soph. 120 Chemistry May 2012 Students working in your lab (previous and present) applying/accepted to graduate school: Student Institutions applying to Area of Study Everette, Jace UALR (completing MS) Chemistry Douglas, Shanek accepted to Med. School in Caribbean Medicine Cade, Charvon UAMS Pharmacology Green, Ashlee University of Texas Education

Wangila Project - Publications Sedrakyan, G.Z., Evans, F.E., Minasyan, S.H., Tavadyan, L.A, Wangila, G.W., Walker, R.B. and Sorenson, J.R.J. “NMR and FTIR Studies of Coordinate Bonding and Intramolecular and Intermolecular Hydrogen Bonding in Zinc (II) (3,5-Diisopropylsalicylate) 2.”Journal of Coordination Chemistry 2008, 61(18) Walker, R.B. and Everette, J.D. “Comparative Reaction Rates of Various Antioxidants with ABTS Radical Cation” Journal of Agricultural and Food Chemistry. 2009, 57(4), , Apostolov EO, Soultanova Z, Savenka A, Bagandov O, Yin X, Stewart AG, Walker R, Basnakian AG. Deoxyribonuclease I is Essential for DNA Fragmentation Caused by Gamma Radiation in Mice. Radiat Res Oct;1 72(4): Everette, J.D. Bryant, Q.M. Green, A.M., Abbey, Y.A. Wangila, G.W. and Walker, R.B. “A thorough Study of Reactivity of Various Compound Classes towards the Folin-Ciocalteu Reagent” Submitted to Journal of Agricultural and Food Chemistry

Wangila Project Presentations Local Presentations Esmeralda Castaneda and Wangila, G.W. A comparative study of vitamin C in commercial tablets and juices, and determination of antioxidant activity using NBT and DPPH assay. Presented at University of Arkansas at Pine Bluff on July 17 th Antoinae Hawkins and Wangila, G.W. Synthesis, characterization and the superoxide dismutase activity of Manganese Di-bromosalicylate and Di-tert- butlysalicylate with NBT Assay. Presented at University of Arkansas at Pine Bluff on July 17 th Regional Presentations Grant W. Wangila, Charvon Cade, Ashlee Green, Richard B. Walker and Alexei Basnakian Copper Complexes as Scavengers of Reactive Oxygen Species and Cell Regeneration in TKPTS Cells. Presented at the annual INBRE 2009 conference held at Fayetteville, Arkansas. Richard B. Walker, Jace D. Everette, Quinton M. Bryant, Ashlee M. Green, Yvonne A. Abbey and Grant W. Wangila. Reactivity of Various Compound Classes Towards the Folin-Ciocalteu Reagent Presented at the 4th Annual BioNanoTox conference, Little Rock Arkansas November 2009 Richard B. Walker, Jace D. Everette, Quinton M. Bryant, Ashlee M. Green, Yvonne A. Abbey and Grant W. Wangila. Reactivity of Various Compound Classes towards the Folin-Ciocalteu Reagent. Presented at the annual INBRE 2009 conference held at Fayetteville, Arkansas National Presentations Grant W. Wangila, Charvon Cade, Ashlee Green, Richard B. Walker and Alexei Basnakian Copper Complexes as Scavengers of Reactive Oxygen Species and Cell Regeneration in TKPTS Cells. Presented at 2009Southeast IDeARegional Meeting conference, Charleston, South Caroline, October 2009 Grant W. Wangila and Richard B. Walker, Jace D. Everette, Quinton M. Bryant, Ashlee M. Green, Yvonne A. Abbey and Reactivity of Various Compound Classes towards the Folin-Ciocalteu Reagent. Presented at 2009Southeast IDeARegional Meeting conference, Charleston, South Caroline, October 2009 Grant W Wangila, Metal Complexes as Scavengers of Reactive Oxygen Species and Cell Regeneration in Prostate Cancer Chemotherapy. University of North Texas Health Science Center (UNTHSC) – At Forth Worth, Annual Health Disparity conference on; May 2009.

Wangila Project Grants PI Institution Type Organization RFA # Total Funds Federal. NIH Health Disparity SP20MD ,000 (funded) Federal The Academy of Applied Sc. G (funded) Non Federal Arkansas Space Grant NA 15,000 (funded)

Wangila Project Equipment Used Biotek 96-well Fluorescent Plate reader $22,000 INBRE Thermo Fisher Nicolet x700 FTIR Spectrometer $30,000 Title III Shimadzu HPLC $50,000 Title III Photodiode-array UV/Visible spectrophotometer $25000 INBRE