for pure liquid samples

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Presentation transcript:

for pure liquid samples IR sample preparation for pure liquid samples

What is a thin film cell? a thin film cell is a drop of pure liquid sample sandwiched between salt (NaCl) discs

Thin film IR cells Salt (NaCl) discs are used To hold the sample So the holder won’t absorb infrared light and only sample absorption is detected

Sample preparation so only dry (anhydrous) samples are used because even traces of water etch the IR discs affecting the path length of light and the optical quality of discs

use clean gloves to work with salt discs put on an extra pair of gloves to protect cells from anything aqueous on your regular gloves

Preparing salt discs Slide off The top of the Desiccator Remove

replace desiccator cover immediately remove discs from sleeves and rest on kim wipe

to clean discs add a few drops of chloroform to each disc rub gently to clean off all traces of sample …

adding sample add one or two drops of sample in the middle of the 1st disc lower the 2nd disc on top as shown to spread sample evenly

placing discs in cell holder use your finger to make a platform in the cell holder lower in the NaCl discs do not wedge them

gently place lid on top slide into position in light beam

close sample compartment remove gloves before using keyboard

to clean discs add a few drops of chloroform to each disc rub gently to clean off all traces of sample …

Keep discs dry replace in desiccator after cleaning …