CavinKare Research Centre Presentation - R&D CavinKare Research Centre CavinKare Pvt. Ltd. Confidential Document : Access to Approved Users Only

Objectives of R&D To contribute to the business objectives of CKPL by developing innovative, cost effective and superior products and processes To make R&D a self-supporting / profit making activity by creating and developing Intellectual Property through out-licensing or national / international collaborations

Product Portfolio Personal Care : Hair, Skin and Home Shampoo Chik, Nyle, Meera, Karthika Hair Wash Powder Meera, Karthika Hair Dye Indica Hair Oil Meera, Nyle Cream / Lotion Fairever, FE Mantra, Nyle CC & ML Talc, Deo & Dab-on Spinz Toilet Bowl Cleaner Tex Mosquito Coil Dishoom Foods : Ready-to-eat Pickles Chinni’s, Ruchi Spices Chinni’s Vermicelli Chinni’s Sweets Ruchi Dry Fruits Chinni’s Moments Snack Bar Chinni’s Cruncho

R&D - Relationships Personal Care (Domestic Business) Salon Business (Trends in Vogue) International Business Personal Care (Domestic Business) Foods (Domestic Business)

R&D Strategy for Competitive Advantage To Provide Superior Products at Affordable Price Points Superior Formulation (sensory experience; delivery of benefit; care) Discovery of new ingredients (exclusivity; superior performance) Developing new / improved processes (cost effectiveness) Developing new technology (exclusivity; superior performance) Strengthening biology (developing capability; studying new pathways) Leveraging IPR (drafting claims; analysis; competitive intelligence)

R&D Set-up & Facilities

CRC at a Glance Buildings = 2 Total Build-up Area = 30000 Ft2 Annual Expenditure = @1% of Turnover Personnel Strength = 40 (Projected 2007) (Technical) Educational Status Number Doctorates 8 Masters 24 Graduates 4 Total 36 Personnel Status (As on Aug 07)

Organisation Chart (2007-08)

Major Equipments (Analytical) Gas Chromatograph HPLC TLC Scanner UV Spectrophotometers Moisture analyser (Infrared, Karl-Fischer) Viscometer Mexameter Skin Testing Equipment (sebum level, moisture, pH, elasticity, TEWL) Hair Testing Equipment (tensile prop., combability, volume) Reflection Spectrophotometer

Major Equipments (Infrastructural) Stability chambers Rotary evaporator Clean air workbench Cold room Ultra-low Temp Fridge (-70°C) Washing Machine Soxhlet extractor Autoclave Tray Driers Cell culture facility Tergetometer Triple Roll Mill Contra-Rotary Mixer Sigma Mixer Simplex Plodder Ribbon Mixer Horizontal Mixer Mini Soap Plodder Rotary coating pan Generator Backup Effluent Treatment Plant

Information / Knowledge Management Library Number of Books = 806 Number of Periodicals Subscribed = 35 Number of Databases Subscribed = 3 (CTFA – online; CTFA-IRDB; STN; Delphion) Number of Libraries to which affiliated = 5 (CLRI, IIT, IISc., BCL & MGR Medical Univ) Membership of Other Organisations / = 2 Professional Bodies (CTFA(USA), BIS)

Information / Knowledge Management Information Systems A fully networked facility with a central control (server) based on ‘thin client’ technology and a Document Management System Sample Tracking for all analytical operations including work flow

R&D Programme

R&D Programme : Basic Research Extraction, isolation and characterisation of bioactive / functional compounds from natural sources Synthesis, characterisation and new process development of ingredients for personal care products Screening of natural products for microbiocidal activities with an emphasis on preservation of products and functional delivery (e.g. antidandruff) Characterisation of major ingredients presently used in personal care products to optimise their performance

R&D Programme : Product Development Development of Formulations and Processes for Safe & Effective Products with emphasis on functional delivery in the areas of - Hair Care Products Skin Care Products Cleaning and Hygiene Products Continuous optimisation and development of new formulation to keep the product cost under control Development of Spices, Vermicelli and Ready-to-Eat Products Such as Pickles, Nut based products (like peanuts, cashew), Snack foods and Traditional Sweets

Outline of R&D Activities

(Selection of Ingredients) Product Development (Selection of Ingredients) Focus on Benefits Delivery of Promise (Claims substantiation) Superior Sensory Experience Gentle / Safe

Product Development : Shampoo Typical Ingredients Typical Analysis

Sensory Evaluation for Products

Product Support Identifying / Developing New Ingredients (biochemistry, microbiology & chemistry) Developing Specifications & Methods (Measurement) Developing New Processes (Chemistry) Demonstrating Efficacy (Biochemistry, Claim substantiation) Facing the Challenge of Contamination (Microbiology) Information / Knowledge Management (Library) Facilitating Activities (IT services, administration etc)

Claim Substantiation Safety evaluation of Products e.g. Irritancy test Developing study protocol Identifying clinical investigator Preparing documents like Consent Form, Investigator Declaration Form, Product Brief, Assessment Form etc Recruitment of volunteers Coordination and conduct of the study Data collation, Analysis and Report submission

Hair Oil - Study Highlights (Claim Substantiation) Note : No. of hairs obtained is the number of hair collected during combing in the clinic

Evaluating a Skin Lightening Cream (Claim Substantiation) Reduction in Average Mexameter Reading = 248 % Reduction = 13.95 % Assessing Reduction in Pigmentation Volunteer Perception of Efficacy

Biochemical Assays Mildness Assay (Shampoo) Skin Lightening Assay Zein assay Collagen swelling pH rise Skin Lightening Assay Tyrosinase inhibition assay DOPA autooxidation inhibition assay Anti-aging Assay Elastase inhibition asssay Antiinflammatory Assay Soyabean lipooxygenase inhibition assay Antioxidant Assay DPPH radical scavenging assay Deoxyribose degradation assay Superoxide Dismutase assay

Cell Based Assays Skin Lightening 1. Melanin assay 2. Tyrosinase inhibition assay Oxidative stress Cytotoxicity Assay Cytotoxicity / Proliferation MTT assay Mildness Assay (Shampoo) 1. Cytotoxicity assay 2. RBC lysis assay Cell lines Available : Melanocyte (melanoma) B16-F10, B16-F1 & G361 Fibroblast (normal) NIH / 3T3 Keratinocyte (carcinoma) A431 General (cancer) HeLa Primary cell lines Fibroblasts & Keratinocytes

Chemistry Extraction, isolation and characterisation of bioactive / functional Compounds from natural sources (skin lightening, antioxidants, antiaging etc.) Synthesis, characterisation and new process development of Ingredients for personal care products Screening of natural products for microbiocidal activities with an Emphasis on preservation of products and functional delivery (e.g. antidandruff) Development of natural hair colours

Chemical Process Oleochemicals Secondary Surfactants Monoethanol amides Amidopropyl betaines Also useful as Thickening Agent

Microbiology Study of anti-microbial efficacy (MIC, synergy etc) Selection of suitable preservatives for products Estimation of microbial load during development Preservative Challenge Test Screening natural products for antidandruff activity Evaluation of competitive products

Measurements Total No. Assays = >190 Major Responsibilities Analysis of Own Products Analysis of Competitor Products Setting up Specifications Interfacing with Regulatory Authorities (BIS, Drug Controllers) Major Responsibilities Sample Tracking

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