St-ABCG1 Colocalizes with a Plasma Membrane Protein St-ABCG1 Colocalizes with a Plasma Membrane Protein.Arabidopsis protoplasts were cotransfected with St-ABCG1-RFP and At-FLS2-YFP. St-ABCG1 Colocalizes with a Plasma Membrane Protein.Arabidopsis protoplasts were cotransfected with St-ABCG1-RFP and At-FLS2-YFP. Expression of the proteins was detected by confocal laser scanning microscopy. The channels for RFP, YFP, and chlorophyll fluorescence are shown, as well as the bright-field and merged images. The experiment was performed twice with similar results. Bars = 5 μm. Ramona Landgraf et al. Plant Cell 2014;26:3403-3415 ©2014 by American Society of Plant Biologists