1. Apical localization of PKA fusion constructs in infected and fully differentiated human airway epithelial cells.
Apical localization of PKA fusion constructs in infected and fully differentiated human airway epithelial cells. Fully differentiated cells were infected in their undifferentiated state with foxj1 promoter-containing lentiviral constructs and stained for cilia with anti-acetylated α tubulin (ac. tubulin) and an Alexa Fluor-555-coupled secondary antibody (red) and with DAPI for nuclei (pseudo-colored in white). (A) Magnified z-axis reconstructions showing expression of both fusion proteins in cilia. (B) Z-axis reconstructions and xy cuts through different levels of the cultures corresponding to cilia, apical and basal cell compartments are shown. Channels are labeled at the bottom including overlay images. Both CFP- and YFP-labeled PKA subunits were mainly localized to the apical compartment of the cells and they can be easily seen inside cilia. Bar, 50 μm.
Andreas Schmid et al. J Cell Sci 2006;119:
© The Company of Biologists Limited 2006