Binding characteristics of mAbs isolated from plasmablasts during acute ZIKV infection. Binding characteristics of mAbs isolated from plasmablasts during.

Slides:

Advertisements

Similar presentations

Fibroblast activation in WT and NFATc2-deficient mice.

Advertisements

Longitudinal analysis of cross-group HA stem–binding lineages.

MP cells established in Rag γc KO mice are Toxoplasma antigen–unspecific T-bet+ population. MP cells established in Rag γc KO mice are Toxoplasma antigen–unspecific.

TPAD controller performance for three force components.

FIP200 maintains microRNA1198-5p expression via Ago2 in naïve T cells.

Cable-driven system diagram for structure matrix.

Specific depletion of CD4-DTR–derived CD4 T cells.

Three different types of transfer functions with a codomain of [0,1].

Splenic CD169+ macrophages express a unique gene profile.

TPAD training protocol.

Tfr cells’ transcriptomic profile distinguishes them from Treg and Tfh cells. Tfr cells’ transcriptomic profile distinguishes them from Treg and Tfh cells.

Longitudinal analysis of ZIKV E–specific memory B cell responses in the DENV-experienced donors. Longitudinal analysis of ZIKV E–specific memory B cell.

Regulatory CD4+ T cell–derived IL-10 is important for B cell differentiation and the GC response. Regulatory CD4+ T cell–derived IL-10 is important for.

Examples of AEGIS autonomous target selection.

Workspace comparison of Delta robots.

Protein sequence alignment of the NS3 helicase–encoding region of 63 flaviviruses demonstrates conservation of a KIR2DS2-binding peptide. Protein sequence.

Ex vivo testing of the soft robotic devices.

Human PBMC-derived MERS-CoV–specific T cells are multifunctional.

Group data during free walking between sessions 1 and 16.

Virus-specific T cell responses are detected in all MERS survivors.

Resistance of CD141+ DCs to HIV and influenza virus infection at the level of viral fusion. Resistance of CD141+ DCs to HIV and influenza virus infection.

VH usage of cross-reactive B cells induced by H5N1 or H7N9 vaccination

Human cells produce type I and III IFNs upon Af stimulation.

Comparison of repertoire distributions to baseline.

Enhanced expression of TLR7 protein in PBMCs from women.

Molecular and functional characterization of antibodies from PfCSP-reactive memory B cells and plasmablasts. Molecular and functional characterization.

Tfr cells robustly secrete IL-10 after acute viral infection.

Tfr cell–derived IL-10 is important for B cell differentiation and the GC response. Tfr cell–derived IL-10 is important for B cell differentiation and.

DC subset cooperation for activation of antiviral T cells.

Macrophage-resident NRP1 mitigates cytokine release and proinflammatory polarization. Macrophage-resident NRP1 mitigates cytokine release and proinflammatory.

Analysis of plasmablast responses in one naïve and three DENV-experienced donors. Analysis of plasmablast responses in one naïve and three DENV-experienced.

Fig. 5 Correlation of RNA expression and protein abundance.

Protein sequence alignment of the NS3 helicase–encoding region of 63 flaviviruses demonstrates conservation of a KIR2DS2-binding peptide. Protein sequence.

Blood Tfr cells are indicators of ongoing humoral activity.

Fig. 2 Transport properties of a BP transistor at low temperature.

CXCR5+/+ TFH cells are essential for the generation of LCMV-neutralizing antibodies and clearance of a persistent LCMV infection. CXCR5+/+ TFH cells are.

Fig. 3 The electrical contact of direct-printed and reconfigured liquid metals. The electrical contact of direct-printed and reconfigured liquid metals.

Fig. 2 Global production, use, and fate of polymer resins, synthetic fibers, and additives (1950 to 2015; in million metric tons). Global production, use,

Evaluation of multidonor class Ig sequences obtained from H5N1 and H7N9 vaccinated donors. Evaluation of multidonor class Ig sequences obtained from H5N1.

CD4-TEMRA cells are heterogeneous across donors.

Fig. 1 Map of water stress and shale plays.

Fig. 1 Examples of experimental stimuli and behavioral performance.

CD4+CLA+CD103+ T cells constitute a unique cell population in human blood. CD4+CLA+CD103+ T cells constitute a unique cell population in human blood. (A)

Fig. 1 Mean and median RCR (Relative Citation Ratio) of Roadmap Epigenomics Program research articles for each year. Mean and median RCR (Relative Citation.

Fig. 2 IT1t prevents TLR7-mediated inflammation in pDCs.

Fig. 2 Stratigraphic profile of the Area 15 excavation block showing the diagnostic cultural materials and components alongside the stratigraphic sequence.

MR1Ts recognized by the hpMR1+EC tetramer are more likely to be TRAV1-2−. MR1Ts recognized by the hpMR1+EC tetramer are more likely to be TRAV1-2−. PBMCs.

Fig. 3 Rotation experiment, setup.

Fig. 1 Product lifetime distributions for the eight industrial use sectors plotted as log-normal probability distribution functions (PDF). Product lifetime.

Fig. 1 Distribution of total and fake news shares.

Fig. 2 2D QWs of different propagation lengths.

MR1Ts respond to microbially derived ligands loaded on hpMR1 tetramers

AEGIS autonomous targeting process.

Examples of AEGIS autonomous target selection.

Functional characterization of multidonor class antibodies.

Fig. 3 Production of protein and Fe(II) at the end of growth correlated with increasing concentrations of ferrihydrite in the media that contained 0.2.

CD4-CTL effectors share TCR clonotypes with CD4-CTL precursors.

Fig. 1 Global occurrences of hydraulic fracturing–induced seismicity and potential models. Global occurrences of hydraulic fracturing–induced seismicity.

Fig. 2 Top 10 countries, ecoregions, conservation hotspots, and KBAs with the largest area of restoration hotspots. Top 10 countries, ecoregions, conservation.

Fig. 5 Comparison of the liquid products generated from photocatalytic CO2 reduction reactions (CO2RR) and CO reduction reactions (CORR) on two catalysts.

Fig. 2 NP characterization.

Fig. 1 Location of the Jirzankal Cemetery.

Fig. 4 Map of δ18OVSMOW in groundwaters of the British Isles (left) and Strontium (87Sr/86Sr) biosphere map of Great Britain (right). Map of δ18OVSMOW.

Fig. 4 CO2 emission changes triggered by the JJJ clean air policy.

Fig. 5. High burdens of AA signature mutations and predicted immunogenicity in Taiwan HCCs. High burdens of AA signature mutations and predicted immunogenicity.

Fig. 4 Spatial mapping of the distribution and intensity of industrial fishing catch. Spatial mapping of the distribution and intensity of industrial fishing.

Fig. 4 Single-particle contact angle measurements.

Fig. 3 Performance of the generative model G, with and without stack-augmented memory. Performance of the generative model G, with and without stack-augmented.

Fig. 2 Comparison between the different reflective metasurface proposals when θi = 0° and θr = 70°. Comparison between the different reflective metasurface.

Neutralizing activity of mAbs isolated from plasmablasts during acute ZIKV infection in DENV-experienced donors. Neutralizing activity of mAbs isolated.

Presentation transcript:

Binding characteristics of mAbs isolated from plasmablasts during acute ZIKV infection. Binding characteristics of mAbs isolated from plasmablasts during acute ZIKV infection. (A) Specificities of the mAbs isolated from one DENV naïve donor and three DENV-experienced donors. Distribution of mAbs that bind to NS1, recombinant E, epitopes expressed only on whole ZIKV, and those for which no specificity could be assigned are shown. The number at the top of each bar represents the total number of mAbs cloned from each donor. (B) Epitope mapping of recombinant E–specific antibodies isolated from three DENV-experienced donors. Pie charts show the distribution of antibodies that bind to epitopes within DIII, within or proximal to the FL on DII, and defined by mAbs ADI-24314 and ADI-24247. 4G2-like FL: Competition with 4G2, WNV E–reactive, and E-FL–sensitive; non-WNV FL: 4G2-competitive, E-FL–sensitive, and WNV E–nonreactive; FL proximal: 4G2 competitive, E-FL–reactive, and WNV E–nonreactive; DIII: Recombinant DIII–reactive; ADI-24314 competitor: 4G2-noncompetitive, ADI-24314–competitive, and E-FL–reactive; ADI-24247 competitor: 4G2-noncompetitive, ADI-24247–competitive, and E-FL–reactive. NC, not characterized because of low binding affinity. (C) Heat map of mAb binding reactivity to ZIKV and DENV1–4 E proteins or whole viral particles (left) and ZIKV and DENV1–4 NS1 proteins (right). The E-specific mAbs shown were isolated from DENV-experienced donors. Apparent binding affinities for recombinant E and NS1 proteins were determined by BLI measurements, and apparent binding affinities for whole virus were determined by ELISA. Thomas F. Rogers et al. Sci. Immunol. 2017;2:eaan6809 Copyright © 2017 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works