In vivo tumorigenicity of MKN-1 cells with sustained suppression of HDAC2. In vivo tumorigenicity of MKN-1 cells with sustained suppression of HDAC2. A, MKN-1 parental, mock, HDAC2 KD1, and HDAC2 KD2 cells (5 × 106) were given subcutaneously into the right flanks of nude mice (n = 9 animals per cell line). Tumor sizes were measured every 3 days for 46 days, as described in “Supplementary Data and Supplementary Materials and Methods”. *, P < 0.01 versus mock cells. B, representative images of individual mice are shown (Top), alongside images of excised tumors (Bottom). C, HDAC2 and p16INK4a protein expression in xenograft tumors was evaluated by Western blot analysis. A typical result of 2 conducted experiments is shown. Jeong Kyu Kim et al. Mol Cancer Res 2013;11:62-73 ©2013 by American Association for Cancer Research