EHop-016 and INK128 treatment of myxofibrosarcoma xenografts in NSG mice. EHop-016 and INK128 treatment of myxofibrosarcoma xenografts in NSG mice. A, effects of the inhibitors on growth of subcutaneous MXF8000 xenografts. The mice were treated with vehicle, EHop-016 at 15 or 20 mg/kg, INK128 at 1 mg/kg, or EHop plus INK128. The tumor volume was monitored by caliper. B, tumor weight at the end of the treatment (day 28; top), and photographs of representative tumors (bottom). C, effects of the inhibitors on lung metastases after tail-vein injection of MXF8000 cells. Mice were treated with the same drug regimens as in A. Tumor in lung was measured by bioluminescent imaging at treatment day 60 (top). The bottom panel shows representative bioluminescent images of the control group and the group treated with the combination of EHop-016 and INK128. D, schematic model of the tumor-specific integrin-α10 pathway working through oncogenic TRIO and RICTOR toward the downstream effectors for growth and survival. Inhibitors are indicated in bold red type. Tomoyo Okada et al. Cancer Discov 2016;6:1148-1165 ©2016 by American Association for Cancer Research