1. 5FU-induced specific activation of CD8+ T cells.
5FU-induced specific activation of CD8+ T cells. A, EL4 tumor-bearing mice were treated with 5FU (or NaCl for controls; 50 mg/kg body weight) and injected (when indicated) with 5 × 106 MDSC isolated from the spleen of tumor-bearing mice. Tumor-infiltrating leukocytes isolated 5 d after 5FU treatment were stimulated with immobilized anti-CD3 alone, anti-CD3 plus killed EL4 cells, or anti-CD3 plus anti-CD28 for 12 h. IFN-γ production in tumor-infiltrating T CD8 (left) or T CD4 (right) lymphocytes, respectively. B, representative FACS showing the effect of 5FU treatment on IFN-γ production by CD8+ T lymphocytes from EL4 tumor infiltrate and its modulation by passive transfer of MDSC. The experiments were performed thrice with identical results (*, P < 0.05).
Julie Vincent et al. Cancer Res 2010;70:
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