Marrow stromal cells for cellular cardiomyoplasty: Feasibility and potential clinical advantages Jih-Shiuan Wang, MDa,b, Dominique Shum-Tim, MDa, Jacques.

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Presentation transcript:

Marrow stromal cells for cellular cardiomyoplasty: Feasibility and potential clinical advantages Jih-Shiuan Wang, MDa,b, Dominique Shum-Tim, MDa, Jacques Galipeau, MD, PhDc, Edgar Chedrawy, MDa, Nicoletta Eliopoulos, PhDc, Ray C.-J. Chiu, MD, PhDa The Journal of Thoracic and Cardiovascular Surgery Volume 120, Issue 5, Pages 999-1006 (November 2000) DOI: 10.1067/mtc.2000.110250 Copyright © 2000 American Association for Thoracic Surgery Terms and Conditions

Fig. 1 The morphology of rat MSCs in in vitro culture. Phase contrast photomicrograph of twice-passaged culture of MSCs just before implantation. Most adherent MSCs are practically fibroblastic in morphology. Scale bar represents 60 μm. The Journal of Thoracic and Cardiovascular Surgery 2000 120, 999-1006DOI: (10.1067/mtc.2000.110250) Copyright © 2000 American Association for Thoracic Surgery Terms and Conditions

Fig. 2 Labeling of rat MSCs in vitro before implantation into the recipient rat hearts. DAPI epifluorescence (blue fluorescence) of twice-passaged culture of MSCs. Note the obvious nuclear and faint cytoplasmic blue fluorescence. Virtually 100% of the cultured cells are labeled by DAPI. Scale bar represents 30 μm. The Journal of Thoracic and Cardiovascular Surgery 2000 120, 999-1006DOI: (10.1067/mtc.2000.110250) Copyright © 2000 American Association for Thoracic Surgery Terms and Conditions

Fig. 3 MSC grafts in isogenic rat recipient hearts 4 days after implantation. A, DAPI epifluorescence (blue fluorescence) photomicrographs. Note the numerous scattered DAPI-labeled cells present in this field. B, Hematoxylin and eosin stain. Consecutive section to that shown in A. Note concordance between dense hematoxylin staining and presence of DAPI epifluorescence. The labeled MSC-derived donor cells show immature appearance with large nucleus-to-cytoplasm ratio. Scale bars represent 30 μm. The Journal of Thoracic and Cardiovascular Surgery 2000 120, 999-1006DOI: (10.1067/mtc.2000.110250) Copyright © 2000 American Association for Thoracic Surgery Terms and Conditions

Fig. 4 MSC grafts in isogenic rat recipient hearts 4 weeks after implantation. A, DAPI epifluorescence (blue fluorescence) photomicrographs. Incorporation of DAPI-labeled cells with the host myocardium. B, Hematoxylin and eosin stain. Consecutive section to that shown in A. Labeled MSC-derived cells have the same structure with that of the surrounding cardiomyocytes. Asterisks show corresponding cross section of a blood vessel in this pair of images (A and B ). Scale bars represent 30 μm. The Journal of Thoracic and Cardiovascular Surgery 2000 120, 999-1006DOI: (10.1067/mtc.2000.110250) Copyright © 2000 American Association for Thoracic Surgery Terms and Conditions

Fig. 5 Expression of sarcomeric myosin heavy-chain molecules of MSC-derived donor cells in specimens taken 6 weeks after implantation. Combined DAPI (blue fluorescence) and Texas Red (red fluorescence) images of the same microscopic field. Arrow shows DAPI-labeled cell is positively stained with sarcomeric myosin heavy-chain immunofluorescence (with use of MF20 primary antibody and Texas Red conjugated secondary antibody), which shows clear striations. Scale bar represents 15 μm. The Journal of Thoracic and Cardiovascular Surgery 2000 120, 999-1006DOI: (10.1067/mtc.2000.110250) Copyright © 2000 American Association for Thoracic Surgery Terms and Conditions

Fig. 6 Expression of connexin 43 of MSC-derived donor cells in a specimen 6 weeks after implantation. Combined DAPI and fluorescein (green fluorescence) image. Anti-connexin 43 immunofluorescence is elicited, by using fluorescein conjugated secondary antibody. Positive connexin 43 staining (arrowheads) is found at the interfaces between 1 DAPI-labeled cell (arrow) and neighboring non-labeled cells (host cardiomyocytes) and between non-labeled cells. Scale bar represents 15 μm. The Journal of Thoracic and Cardiovascular Surgery 2000 120, 999-1006DOI: (10.1067/mtc.2000.110250) Copyright © 2000 American Association for Thoracic Surgery Terms and Conditions