Identification of meiotic and postmeiotic gene expression in testicular tissue of patients histologically classified as Sertoli cell only Pasquale Patrizio, M.D., Stacey M Ricci, B.S., John E Tomaszewski, M.D., Norman B Hecht Fertility and Sterility Volume 74, Issue 4, Pages 785-790 (October 2000) DOI: 10.1016/S0015-0282(00)01515-6
Figure 1 Seminiferous tubules of patient 3. (A), From the right testis and shows only a few residual spermatogonia and spermatocytes. (B), Also from the right testis and spermatogonia are seen as the only germinative epithelium. (C), From the left testis and shows a Sertoli cell-only pattern. Original magnification, ×200. Patrizio. Gene expression in Sertoli cell only. Fertil Steril 2000. Fertility and Sterility 2000 74, 785-790DOI: (10.1016/S0015-0282(00)01515-6)
Figure 2 The PCR amplification of RNA from a right testicular biopsy from patient 3. Contrary to the histologic findings (Fig. 1A and B), PCR amplification of the markers for lactate dehydrogenase C4 (Ldhc4), a meiotic germ cell marker, and TP-1and PRM-1, postmeiotic germ cell markers, were identified. HPRT (hypoxantine phosphoribosyltransferase) represents a control marker for somatic and germ cells. Patrizio. Gene expression in Sertoli cell only. Fertil Steril 2000. Fertility and Sterility 2000 74, 785-790DOI: (10.1016/S0015-0282(00)01515-6)