Ca2+-mediated differentiation of primary mouse keratinocytes is independent of epiplakin. Ca2+-mediated differentiation of primary mouse keratinocytes.

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Ca2+-mediated differentiation of primary mouse keratinocytes is independent of epiplakin. Ca2+-mediated differentiation of primary mouse keratinocytes is independent of epiplakin. (A) Immunoblotting of protein extracts from keratinocytes grown in the presence of 1.3 mM Ca2+ for 1, 3 or 5 days, using antibodies as indicated. Note the upregulation of epiplakin and involucrin (differentiation marker) compared with tubulin (loading control). (B) Primary keratinocytes isolated from wild-type (+/+) and epiplakin-deficient (–/–) mice were grown in the presence of 1.3 mM Ca2+ for the times indicated and then subjected to immunofluorescence microscopy using antibodies as indicated. Scale bar: 10 μm. Daniel Spazierer et al. J Cell Sci 2008;121:825-833 © The Company of Biologists Limited 2008