1. The transcriptional activation activity of PIFs is higher in the cop1-4hfr1 background compared with the cop1-4 background.
The transcriptional activation activity of PIFs is higher inthecop1-4hfr1backgroundcompared withthecop1-4 background. (A) The expression levels of PIF target genes are lower in the pifQ and cop1-4 backgrounds, but higher in the cop1-4 hfr1 background. Total seedling RNA was extracted from 3-day-old dark-grown wild-type Col-0, pifQ, cop1-4 and cop1-4hfr1 seedlings. PP2A was used as an internal control. Wild-type Col-0 was set as 1. Error bars indicate standard deviation (n=3 independent biological repeats). (B) The PIL1 and XTR7 promoter occupancies of TAP-PIF1 were reduced in the cop1-4 background but increased in the cop1-4hfr1 background. ChIP-qPCR assays were performed on 3-day-old dark-grown seedlings expressing TAP-PIF1 fusion protein on cop1-4 and cop1-4hfr1 backgrounds. (C) Photographs of wild-type, cop1-4, cop1-4hfr1, cop1-4/TAP-PIF1 and cop1-4hfr1/TAP-PIF1 seedlings. Seedlings were grown in the dark for 5 days. (D) Bar graph showing hypocotyl lengths of various genotypes as described in C. Error bars indicate standard deviation. Significant differences between different genotypes were determined using one-way ANOVA and Tukey's HSD tests, indicated by different letters. Scale bar: 5 mm.
Vinh Ngoc Pham et al. Development 2018;145:dev169870
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