RT-PCR analysis of different rat brain regions.

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Conversion of mRNA to cDNA by Reverse Transcription

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RT-PCR analysis of different rat brain regions. RT-PCR analysis of different rat brain regions. A, Expression of TASK1-3 and Kir2.3 channels in dLGN. B, Expression of TASK5 channels in dLGN and cerebellum. C, Expression of Kir3.1-3.4 channels and ACh receptors (m2-m3AchR) in dLGN. The sizes of the DNA marker bands are indicated in the left margin. When H2O and brain mRNA not subjected to reverse transcription were used as negative controls instead of cDNA, no PCR bands could be detected because of the lack of adequate substrates for the DNA polymerase (data not shown). Sven G. Meuth et al. J. Neurosci. 2003;23:6460-6469 ©2003 by Society for Neuroscience