1. PCR analysis of platelet cDNA preparations
PCR analysis of platelet cDNA preparations.A, PCR was performed to detect a platelet marker (CD151) and a nucleated cell maker (T cell receptor).
PCR analysis of platelet cDNA preparations.A, PCR was performed to detect a platelet marker (CD151) and a nucleated cell maker (T cell receptor). Lanes 1–5, cDNA from individual platelet preparations. Lane 6, negative (no template) control. Lane 7, cDNA prepared from whole blood. Platelet RNA preparations are free of contaminating cell markers. B, PCR products from the 5′ end of GP IIb and GP IIIa were routinely detected in platelet cDNA synthesized by priming with oligo-dT at the 3′ end, demonstrating intact RNA up to 4 kb long. Lanes 1–11, individual platelet cDNA preparations. Lane 12, no template control. C, The entire coding region of Gas6, amplified from human platelet cDNA. Lane 1, platelet cDNA. Lane 2, no template. Lane 3, molecular weight markers, size indicated in kbp.
J. P. McRedmond et al. Mol Cell Proteomics 2004;3:
© 2004 The American Society for Biochemistry and Molecular Biology