Organoid-derived transplantable corneal epithelial grafts on human amniotic membrane substrate. Organoid-derived transplantable corneal epithelial grafts.

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Organoid-derived transplantable corneal epithelial grafts on human amniotic membrane substrate. Organoid-derived transplantable corneal epithelial grafts on human amniotic membrane substrate. (A) Confocal images of tissue sections of epithelial grafts generated using adult limbal tissues and (B) iPSC-derived corneal organoids on denuded hAM (i). Both the engineered grafts were comparable in terms of spatial distribution and expression patterns of corneal epithelium-specific markers such as PAX6, P63, K12 (ii-iii); cytoskeletal proteins such as K17 (iv), K19 (v) and VIM (vi); and of the basal stem cell marker P75 (vii). Arrows indicate the dual positive basal epithelial cells (ii-v). Arrowheads indicate the PAX6+P63− suprabasal cells (ii). Praveen Joseph Susaimanickam et al. Development 2017;144:2338-2351 © 2017. Published by The Company of Biologists Ltd