Blocking actin turnover affects AJ remodeling and E-cad dynamics.

Slides:



Advertisements
Similar presentations
Rap1 regulates the accumulation of AJ material during ZA morphogenesis
Advertisements

Sec1p requires Boi1/2p for proper localization.
Dual Modes of Cdc42 Recycling Fine-Tune Polarized Morphogenesis
(a) (b) SUPPLEMENTARY FIGURE 1:
Philsang Hwang, Shih-Wei Chou, Zongwei Chen, Brian M. McDermott 
Change in fluorescence / min
Plakophilin 2 Affects Cell Migration by Modulating Focal Adhesion Dynamics and Integrin Protein Expression  Jennifer L. Koetsier, Evangeline V. Amargo,
Volume 19, Issue 11, Pages (June 2017)
Supplementary Figure 1 A. B..
Fig. 1 Labeled endogenous mRNA in MCP×MBS mouse
Scanning electron microscopy analysis of EGK-I to -V chick embryos.
Volume 28, Issue 6, Pages e3 (March 2018)
Volume 23, Issue 12, Pages (June 2013)
Volume 136, Issue 6, Pages (March 2009)
Fig. 3 BX795 blocks the synthesis of HSV-1 virions.
Volume 57, Issue 5, Pages (March 2008)
Volume 89, Issue 5, Pages (November 2005)
Vasudha Srivastava, Douglas N. Robinson  Current Biology 
The β-tubulin C-terminal tail modulates the coordination of microtubule assembly in an isotype-dependent manner. The β-tubulin C-terminal tail modulates.
Zhang-Yi Liang, Mark Andrew Hallen, Sharyn Anne Endow  Current Biology 
Actin Cytoskeleton-Dependent Dynamics of the Human Serotonin1A Receptor Correlates with Receptor Signaling  Sourav Ganguly, Thomas J. Pucadyil, Amitabha.
Nec‐1 inhibits the phosphorylation and aggregation of tau
Dual Modes of Cdc42 Recycling Fine-Tune Polarized Morphogenesis
Marko Kaksonen, Christopher P. Toret, David G. Drubin  Cell 
Axonal swelling and impairment of dendritic development in Purkinje cells from Pex14ΔC/ΔC BL/ICR mouse upon treatment with BDNF. Axonal swelling and impairment.
Translation dynamics of single mRNAs in live cells and neurons
Volume 15, Issue 1, Pages (April 2016)
Fig. 6. Rolling neutrophils extract membranes from fragile remnant PS+ platelets. Rolling neutrophils extract membranes from fragile remnant PS+ platelets.
K. Venkatesan Iyer, S. Pulford, A. Mogilner, G.V. Shivashankar 
LFA-1 is present in cytosolic clusters similar to those containing RhoB and tubulin in migrating T lymphocytes, and reducing RhoB abundance impairs the.
HURP is an obstacle for KIF18A on K-fibers in metaphase cells.
Fig. 2 ALRN-6924 rapidly increases transcription at the p21 locus and affects its bursting dynamics. ALRN-6924 rapidly increases transcription at the p21.
Yuri Oleynikov, Robert H. Singer  Current Biology 
Fig. 3. HECTD2 contains a naturally occurring polymorphism at A19, which mislocalizes to the cytosol. HECTD2 contains a naturally occurring polymorphism.
Yun Wah Lam, Angus I. Lamond, Matthias Mann, Jens S. Andersen 
In Vivo Dynamics of Clathrin and Its Adaptor-Dependent Recruitment to the Actin-Based Endocytic Machinery in Yeast  Thomas M. Newpher, Robin P. Smith,
Wild-type PC1 promotes ciliary localization of endogenous PC2 in ADPKD hepatoblasts. Wild-type PC1 promotes ciliary localization of endogenous PC2 in ADPKD.
Peri‐nuclear Rab34‐positive membranes contact lysosomes
Differential secretion profiles of C. elegans TTR models.
Establishment of monoclonal SMN2-GFP reporter line in HEK293.
Inhibition of DDR1 prevents formation of polarized cysts in 3D.
MMAP is required for spindle dynamics regulation.
Fig. 1. Representative images of the four cell lines using fluorescence microscopy. Representative images of the four cell lines using fluorescence microscopy.
Fig. 1. E-cadherin localizes in nano-scale clusters.
Identification of Z-FA-FMK as a potent compound that increases SMN protein expression in HEK293 and patient fibroblast cells. Identification of Z-FA-FMK.
BMP signalling is dispensable for early gastruloid patterning.
MET/β-catenin complexes during synapse development.
Ventral mouse V-SVZ cells exhibit higher mTORC1 activity than dorsal cells and are responsive to rapamycin. Ventral mouse V-SVZ cells exhibit higher mTORC1.
Remodelling of adult dermis originates from a population of fibroblasts in the hair follicle junctional zone. Remodelling of adult dermis originates from.
TOR, but not OSP, blocks STx2B transport and alters endosomal dynamics
The FRAP mobility of EGFP-UAP56, but not its K95N mutant, is ATP-dependent. The FRAP mobility of EGFP-UAP56, but not its K95N mutant, is ATP-dependent.
EpiDEG efficiently degrades GFP-tagged proteins that localize to different subcellular localizations. epiDEG efficiently degrades GFP-tagged proteins that.
Wnt/β-catenin signalling stabilises and enhances spontaneous symmetry-breaking and polarisation events in gastruloids. Wnt/β-catenin signalling stabilises.
The actin organization and N-cadherin dynamics in migrating cells.
AIP1 localizes in the apical region of preclusters and clusters.
flr mutations result in defects in the adult eye and larval eye disc.
Association of peroxisomes with microtubules is required for proper distribution but not division. Association of peroxisomes with microtubules is required.
Precluster formation as revealed by live imaging and fixed tissue staining. Precluster formation as revealed by live imaging and fixed tissue staining.
FRAP and FLIP analysis of GFP-ADAR2 and GFP-ADAR1C-Term.
Fig. 2. Acetylation stiffens primary cilia.
ChREBPα increases β-cell mitochondrial activity and ATP production in human islets. ChREBPα increases β-cell mitochondrial activity and ATP production.
Fig. 7. Analysis of dFMRP kinetics in dFMRP granules by FRAP
Dynamics of actin-binding proteins in the ICAM-1 complex.
Fluorescence staining of F-actin in cultured astrocytes isolated from wild-type, MMP-2 null, and MMP-9 null mice using Texas Red-conjugated phalloidin.
Subcellular distribution of Mst4 and aPKCι in in vivo enterocytes.
Ribosomal RNA and β-catenin mRNA localize to newly formed presynaptic terminals. Ribosomal RNA and β-catenin mRNA localize to newly formed presynaptic.
Fig. 3 Hydrostatic pressure induces intracellular calcium increase via TRPM7 activation. Hydrostatic pressure induces intracellular calcium increase via.
Nicotine stabilizes GluA1s on dendritic spines in hippocampal cell culture. Nicotine stabilizes GluA1s on dendritic spines in hippocampal cell culture.
Cofilin-GFP forms rods that sequester pMAP during ATP depletion.
Fig. 3 Three small molecules inhibit the transport of CCR5 and do not affect transport of CCR1 or CXCR4. Three small molecules inhibit the transport of.
Presentation transcript:

Blocking actin turnover affects AJ remodeling and E-cad dynamics. Blocking actin turnover affects AJ remodeling and E-cad dynamics. (A-C′) An eye disc cultured in DMSO control (A,A′) shows normal E-cad-GFP signal, whereas Lat-A (B,B′) or Jasp (C,C′) treatment each results in different AJ defects. The boxed MF-proximal regions in A-C are enlarged in A′-C′. Arrow indicates MF. (D-G) FRAP analysis of E-cad-GFP for precluster AJs. (D-F) Colored boxes indicate the region of interest (ROI), where photobleaching is applied and fluorescence intensity is then measured every second for 60 seconds. (G) Recovery curves for DMSO-treated, Jasp-treated and flr mosaic (red) eye discs. For each data point the s.e.m. is shown. See also Fig. S7 in the supplementary material. Scale bars: 20 μm in A-C; 10 μm in A′-C′; 1 μm in D-F. Dandan Chu et al. Development 2012;139:3561-3571 © 2012.