1. Change in fluorescence / min
pH-GluR2L T912A
pH-GluR2L T912D
pH-GluR2Lwt
Change in fluorescence / min
Supplementary Figure 11: Status of the novel T912 site does not affect initial rates of pHGluR2L
internalization induced by NMDA treatment of cultured neurons. The indicated constructs were
expressed in primary hippocampal neurons as in Fig 6 and treated with NMDA. The initial rate of
internalization (which is approximately linear, see Fig 6c-f) for each construct.was plotted as mean + S.E.M.
pHGluR2Lwt, N=8; pHGluR2L T912A, N=5; pHGluR2L T912D, N=4.