Immunological and Statistical Studies of Anti-BP180 Antibodies in Paraneoplastic Pemphigus  Atsunari Tsuchisaka, Hideo Kawano, Atsushi Yasukochi, Kwesi Teye, Norito Ishii, Hiroshi Koga, Ryosuke Sogame, Ayaka Ohzono, Rafal P. Krol, Tamihiro Kawakami, Minao Furumura, Chika Ohata, Xiaoguang Li, Takashi Hashimoto  Journal of Investigative Dermatology  Volume 134, Issue 8, Pages 2283-2287 (August 2014) DOI: 10.1038/jid.2014.151 Copyright © 2014 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Clinical, histopathological, and immunological features. Clinical tense blisters (a) and histopathological subepidermal blister (b). Direct immunofluorescence (IF) for IgG (c) and C3 (d). Indirect IF of human skin (e) and split skin (f, g). Scale bars=50 μm. (h-k) IB results for all paraneoplastic pemphigus patients (lanes 1–59) and two normal sera (lanes N1 and N2). (h) Normal-human epidermal extracts. BP serum reacted with BP230 and BP180 (lane P). Black and blue asterisks, positive for BP230 and BP180, respectively. EPL, envoplakin; IB, immunoblotting; PPL, periplakin. (i) HaCaT. BP serum reacted with LAD-1 (lane P). Red numbers, positive. (j) BP180 NC16a domain RP with positive reactivity by BP serum (lane P). Red numbers, positive. (k) BP180 C-terminal domain RP with positive reactivity with mucous membrane pemphigoid serum (lane P). Red numbers, positive. Journal of Investigative Dermatology 2014 134, 2283-2287DOI: (10.1038/jid.2014.151) Copyright © 2014 The Society for Investigative Dermatology, Inc Terms and Conditions