HSP90β interacts with HNF4A to regulate protein half-life.

Slides:

Advertisements

Similar presentations

Sec1p requires Boi1/2p for proper localization.

Advertisements

Vegetal RNAs antagonise the dorsalising and BMP-inhibiting activity of Vrtn. Vegetal RNAs antagonise the dorsalising and BMP-inhibiting activity of Vrtn.

SESN2 expression in lungs from healthy donors, habitual smokers without COPD and individuals with late-stage COPD. (A) Representative western blots of.

Phosphorylation of serine 73 targets SREBP-1c for ubiquitination and proteasomal degradation Phosphorylation of serine 73 targets SREBP-1c for ubiquitination.

MEL23 and MEL24 inhibit Mdm2 and p53 ubiquitination in cells.

by Sansana Sawasdikosol, Kristin M. Russo, and Steven J. Burakoff

Fig. 5. Wisper is associated with TIA1-related protein and regulates lysyl hydroxylase 2 expression. Wisper is associated with TIA1-related protein and.

UCHL1 Regulates Melanogenesis through Controlling MITF Stability in Human Melanocytes Eun Young Seo, Seon-Pil Jin, Kyung-Cheol Sohn, Chi-Hyun Park, Dong.

Expression of SYCE2 inhibits the interaction of HP1α with H3K9me3.

Overexpression of HGF decreases MET protein levels.

Figure 2 The K19del mutation affects the expression and solubility of CHP1 The K19del mutation affects the expression and solubility of CHP1 (A) Western.

Alpha-synuclein induces autophagy in microglial cells.

Fig. 1. Chlamydia infection causes elevated levels of sortilin.

TP53 western blot of primary cultured tail cells from both wild-type (WT) and Tp53Δ11/Δ11 (−/−) rats. TP53 western blot of primary cultured tail cells.

Fig. 4. Increased adipocyte differentiation in Cbx7-KO ES cells

Expression of tomoregulin-1 in wild-type and cardiac hypertrophy mouse myocardium. Expression of tomoregulin-1 in wild-type and cardiac hypertrophy mouse.

Fig. 1. Coilin interaction with the telomerase complex protein dyskerin is mediated by hTR. Coilin interaction with the telomerase complex protein dyskerin.

TWEAK downregulates SIRT3 in cultured tubular cells and this is prevented by crotonate. TWEAK downregulates SIRT3 in cultured tubular cells and this is.

Fig. 4. Detection of dFMR1 mRNA in dFMRP granules by FISH

Effect of Z-FA-FMK on the expression of SMN proteins in SMA patient iPSCs and iPSC-derived motor neurons. Effect of Z-FA-FMK on the expression of SMN proteins.

Characterisation of eSRS21 protein using an anti-eSRS21 antibody.

Fig. 6. Effect of SAHA and ML on histone acetylation, BAX, and p21CDKN1A expression.PANC-1 and BxPC-3 cells were incubated for 48 hours with 5 µM.

Fig. 5. Receptor tyrosine kinase activation in response to growth factor stimulation. Receptor tyrosine kinase activation in response to growth factor.

Fig. 6. CBX7 expression in adipocyte differentiation of adipose-derived stem cells.(A) The adipose-derived stem cells, ADS1, were analyzed for the capability.

FAK tyrosine phosphorylation is unaffected by loss of LAR activity.

ICAM-1 forms distinct complexes with filamin B, α-actinin-4 and cortactin. ICAM-1 forms distinct complexes with filamin B, α-actinin-4 and cortactin. (A)

The interaction of SYCE2 and HP1α is not modified in response to exogenous DNA damage induced by x-ray irradiation. The interaction of SYCE2 and HP1α is.

Fig. 7. Ror-GFP binds to Myc-tagged Wnts and Wnt receptors.

Fig. 1. Mitotic arrest results in differential RNA association with coilin.Untreated or nocodazole treated HeLa cell lysate was used for RNA immunoprecipitations.

GFP–Sec61b mRNA competes with t-ftz mRNA for the ribosome-binding sites on the ER. (A,B) COS7 cells were transfected with plasmid containing a test gene.

MiR-200c/141 overexpression in LS-8 cells induces E-cad expression and cell-cell adhesion. miR-200c/141 overexpression in LS-8 cells induces E-cad expression.

Distribution and extent of expression.

An inhibitor of HSP90β reduces the level of HNF4A protein in hepatic progenitor cells. An inhibitor of HSP90β reduces the level of HNF4A protein in hepatic.

Functional classification and visualization of differentially expressed genes. Functional classification and visualization of differentially expressed.

USP15 Negatively Regulates Nrf2 through Deubiquitination of Keap1

TWEAK increases histone crotonylation in kidney tubular cells.

2DG downregulates the expression of HIF-1α, PDK1 and c-Myc in NB xenograft. 2DG downregulates the expression of HIF-1α, PDK1 and c-Myc in NB xenograft.

Effect of Dipy on the expression of chondrogenic markers.

Deletion of Sox2 in adult mice inhibits incisor regeneration.

Stability of SsbA (A) and GFP (B) proteins in UA159 and ΔclpX strains.

Cdc2 expression in cultured Schwann cells.

Graf-dependent GEEC endocytosis is required for EGFR internalization and degradation at high Spi. Graf-dependent GEEC endocytosis is required for EGFR.

Impact of LAMP interaction on TAPL function.

Stimulation of cells with FFAR4 agonist.

Kinetics of GFP secretion from CHO cells.

miR-145 regulates IGF1R expression.

BRG1 interacts with RAD52 and regulates its accumulation at DSB sites during homologous recombination repair. BRG1 interacts with RAD52 and regulates its.

Fig. 2. Acetylation stiffens primary cilia.

The influence of SHIP2 on cell migration in breast cancer cells.

The vps13 mutants are hypersensitive to SDS but cell wall integrity is not compromised. The vps13 mutants are hypersensitive to SDS but cell wall integrity.

Running on the treadmill prolonged TrkB activation induced by exogenous BDNF in vivo. Running on the treadmill prolonged TrkB activation induced by exogenous.

Fig. 1. p85β localizes at adhesion plaques and associates with FAK

Kinetics of BDNF-induced Erk, Akt and PLCγ activation in the presence of 15 mM NaCl or 15 mM KCl. Representative western blots (A) and quantitative plots.

EPLIN also plays a crucial role in the apical extrusion of RasV12-transformed cells. EPLIN also plays a crucial role in the apical extrusion of RasV12-transformed.

Expression of IP3R subtypes in HEK cells.

BCL-3 regulates expression of stemness-associated Wnt targets.

Vps36 interacts with Smo in the absence of Hh

GOLPH3 interacts with COG complex proteins.

Exosomal transfer of sortilin to a target cell.

Ctip2 directly regulates Notch1 expression in differentiated cells.

APLF regulates genes implicated in MET during the generation of iPSCs from fibroblasts. APLF regulates genes implicated in MET during the generation of.

Mutant MFN2 proteins have normal half-lives and are properly localized to mitochondria. Mutant MFN2 proteins have normal half-lives and are properly localized.

Recognition of naturally processed HTLV-1 Tax protein derived from HAM patient's PBMC. A, HTLV-1 Tax protein content was evaluated by Western blot analysis.

Fig. 8 C9orf72 knockdown results in an increase in autophagic flux.

PVT1 binds to the FOXM1 protein and increases its protein level by enhancing its stability. PVT1 binds to the FOXM1 protein and increases its protein level.

AS1411 alters subcellular distribution of PRMT5 in a time-dependent, dose-dependent, and nucleolin-dependent manner. AS1411 alters subcellular distribution.

Fig. 6 The C9ORF72/SMCR8 complex regulates ULK1.

Bid associates with TRAF6 in microglia and astrocytes, as shown by coimmunoprecipitation and PLA. A, Coimmunoprecipitation of Bid and TRAF6 in BV-2 cells.

Inhibition of HDACs disrupts DNMT1 association with Hsp90.

TCTP enhances the protein stability of Pim-3 by blocking the ubiquitin–proteasome degradation of Pim-3. TCTP enhances the protein stability of Pim-3 by.

Presentation transcript:

HSP90β interacts with HNF4A to regulate protein half-life. HSP90β interacts with HNF4A to regulate protein half-life. (A) Bar graph showing relative levels of HNF4A mRNA in HSP90β+/+ and HSP90β−/− cells at day 8 of differentiation (mean±s.e.m.). (B) Western blots of cycloheximide (CHX) chase analyses of HNF4A protein in day 8 hepatic progenitor cells derived from HSP90β+/+ and HSP90β−/− iPSCs. Samples were collected at 0, 3, 6, 12, 24, 36, 48 and 60 h after incubation of cells with 100 µM CHX to inhibit protein synthesis. (C) Quantification of HNF4A protein levels by densitometry of western blots performed on CHX chase samples. The level of HNF4A was calculated at each time point and expressed relative to the level of HNF4A in untreated cells (0 h). (D) Western blots to detect HNF4A and HSP90β after immunoprecipitation of HSP90β+/+ and HSP90β−/− day 8 hepatic progenitor cell lysates with an HSP90β antibody. Wild-type iPSC-derived hepatic progenitor cell lysate was used to measure total HNF4A and HSP90β, and immunoprecipitation of the same lysate using IgG was used as a negative control. Ran Jing et al. Development 2017;144:1764-1774 © 2017. Published by The Company of Biologists Ltd