Protection of myocardium by cyclosporin a and insulin: in vitro simulated ischemia study in human myocardium Aviva Schneider, MS, Niv Ad, MD, Uzi Izhar,

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Presentation transcript:

Protection of myocardium by cyclosporin a and insulin: in vitro simulated ischemia study in human myocardium Aviva Schneider, MS, Niv Ad, MD, Uzi Izhar, MD, Igor Khaliulin, PhD, Joseph B Borman, MD, Herzl Schwalb, PhD The Annals of Thoracic Surgery Volume 76, Issue 4, Pages 1240-1245 (October 2003) DOI: 10.1016/S0003-4975(03)00830-0

Fig 1 Protection of human right atrial sections with cyclosporin A (CSA). The atrial sections were exposed to 30 minutes of aerobic equilibration, 90 minutes of simulated ischemia (37°C), and 90 minutes of reoxygenation (untreated control and CSA treated groups). The CSA (0.2 μmol/L) was administered during the preischemic equilibration. Postischemic viability was measured by MTT (3-[4.5 dimethylthiazol 2-yl]-2,5-diphenyltetrazolium bromide). (a) The group includes all the patients (n = 20) mean ± SEM. *p less than 0.001 versus the untreated control group (without CSA). (b) Subgroup analysis of the effect of presurgery oral glybenclamide on the CSA protective effect. Left: atrial slices from patients orally treated with glybenclamide (n = 8). Right: atrial slices from patients nontreated with glybenclamide (n = 12). *p less than 0.001 versus the untreated control group (without CSA). The Annals of Thoracic Surgery 2003 76, 1240-1245DOI: (10.1016/S0003-4975(03)00830-0)

Fig 2 Inhibition of the protective effect of cyclosporin A (CSA) in human right atrial sections. The experimental protocol was the same as in Figure 1; however, atrial slices obtained from a different group of patients without history of diabetes and glibenclamide treatment were used. The KATP channel inhibitor, 5-hydroxydecanoate (5HD, 0.1 mmol/L), was added during the preischemic equilibration (n = 6). *p less than 0.001. The Annals of Thoracic Surgery 2003 76, 1240-1245DOI: (10.1016/S0003-4975(03)00830-0)

Fig 3 Protection of human right atrial sections with insulin. Experimental protocol of simulated ischemia and reoxygenation as described for Figure 1. (a) Insulin (5 mU/mL) was added during preischemia or reoxygenation or both. *p less than 0.001 versus untreated control. #p less than 0.01. ##p less than 0.001 versus insulin preischemia plus reoxygenation. ∧p less than 0.05 versus untreated control. (b) Subgroup analysis of the effect of presurgery oral glybenclamide on the insulin (added during preischemia and reoxygenation) protective effect in left atrial slices (n = 5). *p less than 0.05 versus the untreated control group. The Annals of Thoracic Surgery 2003 76, 1240-1245DOI: (10.1016/S0003-4975(03)00830-0)