p-S6 S240/244 is observed across the V-SVZ lineage.

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p-S6 S240/244 is observed across the V-SVZ lineage. p-S6 S240/244 is observed across the V-SVZ lineage. (A) Graphs displaying p-S6 S240/244 fluorescence intensity levels by flow cytometry across each of the indicated cell lineages. Bars represent the median value. Left: standard scale and right: arcsinh scale. Paired t tests, qNSCs (P = 0.8797), aNSCs (P = 0.0193), TAPS (P = 0.3370), and neuroblasts (P = 0.7523). For all graphs, bars represent mean ± SD. (B) Representative tilescan of mouse P30 V-SVZ with boxed areas highlighted to the right, DAPI (blue), DCX (red), p-S6 S240/244 (green). Scale bars, 100 μm (tilescan), 10 μm (63×). Violin plot displays distribution of mean fluorescence values as measured in ImageJ of p-S6 S240/244-positive, DCX-negative cells with the white bar representing the median. N = 3 mice, three V-SVZ sections per mouse, 365 dorsal, and 235 ventral cells total. Wilcoxon signed rank test, P < 0.0001. (C) Tilescan image of GW33+0 human brain: DAPI (blue), vimentin (green), and DCX (red) with boxed areas highlighted to the right. SB = 2 mm and 200 μm, respectively. p-S6 S240/244 signal intensity is displayed on the rainbow LUT with the edge of the V-SVZ outlined in yellow. Gabrielle V Rushing et al. LSA 2019;2:e201800218 © 2019 Rushing et al.