Figure S3. Zhang et al. A B Myc-SIRT1 - + sir2a+/+ sir2a-/-

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Figure S3. Zhang et al. A B Myc-SIRT1 - + sir2a+/+ sir2a-/- Empty vector + - IB anti-SIR1(10E4) sir2a IB anti-SIRT1(10E4) SIRT1 IB anti-b-actin b-actin IB anti-b-actin b-actin Figure S3. Characterization of a SIRT1 monoclonal antibody, 10E4, in Western blotting analyses. (A) 293T cells were transfected with either empty vector or Myc-SIRT1, thirty six hours after transfection, cells were lysed and anti-SIRT1 Western blotting analysis was performed by anti-SIRT1 antibody, 10E4. The blot was also probed with anti-b-actin antibody to ensure the protein loading. (B)sir2a+/+ and sir2a-/- MEFs were lysed and Western blotting analyses were performed as (A).