Chapter 12-1 DNA Part 1.

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Chapter 12-1 DNA Part 1

I. Griffith and Transformation (1928) A. Griffith needed to know which bacteria caused pneumonia so he could to develop vaccine. B. He isolated 2 strains of bacteria 1. Smooth/encapsulated bacteria 2. Rough/non-encapsulated bacteria

C. Experiment: He isolated both strains and injected them into mice. 1. Smooth bacteria- injected into mice 2. Rough bacteria- injected into mice mice die IS THERE A WAY TO KILL BACTERIA? mice live

Which bacteria strain causes pneumonia ____________ Which bacteria strain that does not cause pneumonia _______________ Smooth Bacteria Rough Bacteria

3. Heat killed smooth bacteria- injected into mice 4. Mixed heat killed smooth bacteria with rough bacteria- injected into mice mice live mice die X X

5. Conclusion: The rough (harmless) strain of bacteria had been transformed into the smooth (disease-causing) strain. A transformation had occurred. 6. Griffith thought the transforming factor might be a gene.

Experiment 3 Experiment 4 Experiment 1 Experiment 2 Heat killed smooth Inject mouse with _______________ bacteria Inject mouse with ________________ And ____________ bacteria Inject mouse with ________bacteria Inject mouse with ________bacteria Heat killed smooth Heat killed smooth rough smooth rough Lives  Dies  Lives  Dies  Mouse _________ Mouse _________ Mouse _________ Mouse _________

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