Proteomic analysis of invasive TCCs

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Proteomic analysis of invasive TCCs Proteomic analysis of invasive TCCs. [35S]Methionine-labeled proteins synthesized in a normal urothelium specimen (cyst 189) and an invasive TCC (566-1 T2-4 G3) were separated by 2D PAGE (isoelectric focusing, IEF) and visualized by autoradiography. Proteomic analysis of invasive TCCs. [35S]Methionine-labeled proteins synthesized in a normal urothelium specimen (cyst 189) and an invasive TCC (566-1 T2-4 G3) were separated by 2D PAGE (isoelectric focusing, IEF) and visualized by autoradiography. The position of actin is given as a reference. The framed area in the gel, corresponding to the portion of the gel containing the 14-3-3 β, σ, η, and ζ isoforms, is shown enlarged in the lower panels of the figure, with the positions for the respective isoforms indicated. José M. A. Moreira et al. Mol Cell Proteomics 2004;3:410-419 © 2004 The American Society for Biochemistry and Molecular Biology