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Manual assessment of the quality of peptide spectra with scores ranging from 5 to 10 of OFFGEL electrophoresis fractions 3 and 4 that were rejected by.

Published byΑδελφά Μελετόπουλος Modified over 5 years ago

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Presentation on theme: "Manual assessment of the quality of peptide spectra with scores ranging from 5 to 10 of OFFGEL electrophoresis fractions 3 and 4 that were rejected by."— Presentation transcript:

1 Manual assessment of the quality of peptide spectra with scores ranging from 5 to 10 of OFFGEL electrophoresis fractions 3 and 4 that were rejected by autovalidation criteria. Manual assessment of the quality of peptide spectra with scores ranging from 5 to 10 of OFFGEL electrophoresis fractions 3 and 4 that were rejected by autovalidation criteria. From a total of ∼1200 spectra 415 were inspected. Peptide spectra that have deviations of the predicted pI value from the average fraction pI within the size of the pH segment covered by one well (i.e. 0 ± 0.13 pH units) are considered in a; all others are shown in b. Numbers on top of the bars in a refer to the numbers of good quality spectra found in the respective score range; the corresponding percentage of the total number of spectra is given in parentheses. Patric Hörth et al. Mol Cell Proteomics 2006;5: © 2006 The American Society for Biochemistry and Molecular Biology

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