Single type II fibers visualized by intracellular labeling.

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Single type II fibers visualized by intracellular labeling. Single type II fibers visualized by intracellular labeling. A, Apical turn of a young (P8) rat organ of Corti with biocytin-filled type II fiber after streptavidin–peroxidase reaction. Scale bar, 125 µm. B, Higher magnification of boxed areas in A, showing trajectory and terminal branches. C, Biocytin-filled type II fiber reacted with streptavidin-Alexa Fluor 488 (green). OHC nuclei labeled with DAPI (blue). Magnifications show en passant (red arrows) and terminal (white arrowheads) swellings of branches from boxed regions. D, Biocytin-streptavidin-Alexa Fluor 488-filled fiber combined with FM1-43-labeled OHCs (red). Magnifications show terminal branches enwrapping the base of outer hair cells. E, Biocytin-streptavidin-Alexa Fluor 488-filled fiber combined with a CtPB2 immunolabel (red). Magnifications show an approximation of some terminal branches to CtBP2 puncta. F, Combined immunolabel for PSD-95 (green) and CtBP2 (red) among OHCs of young rat cochlea. Magnification shows pearl chain pattern found in adult cochlea. Scale bars: B–G, 5 µm; magnifications, 2.5 µm. Rodrigo Martinez-Monedero et al. eneuro 2016;3:ENEURO.0078-16.2016 ©2016 by Society for Neuroscience