Localization of selected clones in mammalian COS-7 cells.

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binding sites 58 of the 473 unambiguously assigned phosphorylation sites are predicted by Scansite to be sites for binding. 50 of these correspond.

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Phosphorylation and sequence disorder in microtubule-associated protein Tau.A, schematic illustration of the domain profile of Tau with all known phosphorylation.

Distribution of phosphorylation sites identified in the cytosolic phosphoproteome.A, numbers of approved phosphopeptides, previously phosphorylated peptides,

Expression of retinitis pigmentosa protein RP1 in human bronchial epithelial cells. Expression of retinitis pigmentosa protein RP1 in human bronchial epithelial.

Percentage of proteins identified in envelope membrane extracts according to the purification method and the number of transmembrane domains. Percentage.

Differential conjugation of endogenous SUMO-1 and endogenous SUMO-2/3 to target proteins.A and B, SUMO-1 and SUMO-2 proteins were produced in E coli and.

Plasma CEP markers stratified by demographic and health factors.

Plasma CEP adducts and autoantibodies by donor age.

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Frequency distribution of the GRAVY of the theoretical proteins (open bars) and of 110 genes encoding proteins identified on a 2-D electrophoresis gel,

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Assay of NOS activity. Assay of NOS activity. Box show total NOS activity, the calcium-dependent activity of the constitutive isoforms of NOS (eNOS and.

Expression of apocrine markers at various stages of apocrine carcinoma progression. Expression of apocrine markers at various stages of apocrine carcinoma.

Relative abundance of proteins identified in MALDI IMS

Success rates in validation of antibodies from external providers

Results from the Morris water task.

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The evolutionary conservation of the phosphoproteomes.a, E. coli. b, B. subtilis. The evolutionary conservation of the phosphoproteomes.a, E. coli. b,

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Colonopshere-enriched proteins display functional interactions.

Proteins previously reported in published MALDI IMS studies and their frequency of observation in the present study. Proteins previously reported in published.

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Sequence similarity clusters of SET domain methyltransferases.

High correlation of expression changes of NMD-regulated genes identified by both the pSILAC screen and previously reported global RNA screens after UPF1.

BIRC6 confers resistance against cisplatin and oxaliplatin.

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A, Western blot analysis of fetuin-A in AGA (lanes 1–5 and 10–13) and IUGR (lanes 6–9, 14, and 15) UC plasma samples. A, Western blot analysis of fetuin-A.

Testing the effectiveness of the three-step peptide fractionation method.A, μLC mass chromatograms of SCX fractions for an acidic FFE fraction. Testing.

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Putative targets of miRNAs directly induced by p53 with down-regulated mRNA- and de novo protein synthesis or reduced de novo protein synthesis only. Putative.

Interaction networks of the regulated phosphoproteins.

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Distribution of the phosphoproteins based on GO analysis, including biological process (Left) and cellular component (Right). Distribution of the phosphoproteins.

A, Absolute ion intensities of m/z 322, 922 and 1522 as function of the transfer time. A, Absolute ion intensities of m/z 322, 922 and 1522 as function.

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Bar plot representation of the transcriptomic changes in Δsaci_ptp and Δsaci_pp2a. Bar plot representation of the transcriptomic changes in Δsaci_ptp and.

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Phylogenetic trees of the closest eukaryotic homologs of clones AY and AY Phylogenetic trees of the closest eukaryotic homologs of clones.

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Occurrence of fur−-only genes and expected sensitivity to Fur.

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Localization of selected clones in mammalian COS-7 cells. Localization of selected clones in mammalian COS-7 cells. Clones lacking predicted membrane-targeting signals or known homologs were transferred to the pCDNA3 mammalian expression vector. The resulting plasmids were co-transfected to COS cells together with p75 as a plasma membrane marker. Cells were fixed after 36 h and immunostained for p75 (green) and Ras (red). Yellow indicates co-localization. Hanna Jaaro et al. Mol Cell Proteomics 2005;4:328-333 The American Society for Biochemistry and Molecular Biology