Prostate-specific LV-Cre/Luci delivery results in focal disease.

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Prostate-specific LV-Cre/Luci delivery results in focal disease. Prostate-specific LV-Cre/Luci delivery results in focal disease. A, live and post-mortem images of LV-Cre/Luci–injected PtenloxP/loxP;Trp53loxP/loxP mouse 69 days after injection. Dissection reveals similar distribution to that shown in Fig. 1C. The luminescence signal was seen in LV-Cre/Luci–injected prostate and seminal vesicle. B, PCR analysis confirms recombination of Pten and Trp53 in LV-Cre/Luci–injected PtenloxP/loxP;Trp53loxP/loxP specifically in the injected right (Rt) anterior prostate (AP). C, IHC analysis of LV-Cre/Luci–injected PtenloxP/loxP;Trp53loxP/loxP prostate reveals a focal lesion in the luminal epithelium of one gland (H&E), which also displays increased proliferation, specific to epithelial cells (Ki-67). Loss of Pten expression, activation of Akt (pAkt), and increased proliferation are also found in this region (middle), which is a unique focus of disease in the cross-section of the entire anterior prostate (right). Note that surrounding stroma remains unaffected in all panels. Scale bars, 100 μm (top right) and 50 μm (all other panels). GU, genitourinary. Hyejin Cho et al. Cancer Discovery 2014;4:318-333 ©2014 by American Association for Cancer Research