1. Adeno-associated Virus Serotype Vectors Efficiently Transduce Normal Prostate Tissue and Prostate Cancer Cells 
Jianzhong Ai, Dan Wang, Qiang Wei, Hong Li, Guangping Gao 
European Urology 
Volume 69, Issue 1, Pages (January 2016)
DOI: /j.eururo
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2. Fig. 1
rAAV6.2 and rAAV7 efficiently transduced mouse prostate following intraprostate injection. (A) Quantification of transduction efficiency in AP (yellow bars) and DLP (orange bars) following intraprostate injection with rAAV vectors of different serotypes expressing EGFP. EGFP fluorescence intensity of cryosections is presented in arbitrary units (a.u.) (B) Representative fluorescence images of AP and DLP cryosections showing merger of EGFP native fluorescence (green) and nuclear staining by DAPI (blue) following injection of PBS, rAAV6.2, or rAAV7. Scale bars, 25μm. (C) Biodistribution of rAAV genomes in AP (yellow bars) and DLP (orange bars) following intraprostate injection of rAAV6.2 and rAAV7. Data are presented as rAAV genome copies per diploid genome. (D) Representative images of immunofluorescence staining of prostate luminal cells (top panels), basal cells (middle panels), and stromal cells (bottom panels), marked by K8, K5, and α-actin staining, respectively. Native EGFP fluorescence images and merged images with DAPI staining (blue) for the same sections are also shown. Arrows indicate representative co-localization of EGFP and cell-type marker signals. (E) Quantification of the percentage of EGFP-positive cells for each cell type. rAAV = recombinant adeno-associated virus; AP=anterior prostate; DLP=dorsal lateral prostate; EGFP=enhanced green fluorescent protein; DAPI=4′,6-diamidino-2-phenylindole.
European Urology  , DOI: ( /j.eururo )
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