A, Absolute ion intensities of m/z 322, 922 and 1522 as function of the transfer time. A, Absolute ion intensities of m/z 322, 922 and 1522 as function.

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Presentation transcript:

A, Absolute ion intensities of m/z 322, 922 and 1522 as function of the transfer time. A, Absolute ion intensities of m/z 322, 922 and 1522 as function of the transfer time. The maxima of the distributions for each m/z value are the optimal times for efficient transfer into the flight tube. B, Fragment spectrum of the iTRAQ labeled peptide LFTGHPETLEK without (blue) and with (red) transfer time stepping that adds the lower m/z range including the reporter ions without sacrificing intensity in the higher mass range. Scarlet Beck et al. Mol Cell Proteomics 2015;14:2014-2029 © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.