Volume 124, Issue 7, Pages 1846-1854 (June 2003) Helicobacter pylori binds von Willebrand factor and interacts with GPIb to induce platelet aggregation  Michael F Byrne, Steven W Kerrigan, Paul A Corcoran, John C Atherton, Frank E Murray, Desmond J Fitzgerald, Dermot M Cox  Gastroenterology  Volume 124, Issue 7, Pages 1846-1854 (June 2003) DOI: 10.1016/S0016-5085(03)00397-4

Figure 1 Platelet aggregation in response to H. pylori. (A ) The effects of H. pylori strains 60910 and J104 on platelet aggregation. (B) The effect of anti-GPIb antibodies on H. pylori-induced platelet aggregation. (C ) Probable binding sites for the anti-GPIb antibodies.28,29 Gastroenterology 2003 124, 1846-1854DOI: (10.1016/S0016-5085(03)00397-4)

Figure 2 Platelet aggregation in platelets from a patient with Bernard-Soulier disease. Response to arachidonic acid and H. pylori. Gastroenterology 2003 124, 1846-1854DOI: (10.1016/S0016-5085(03)00397-4)

Figure 3 Binding of H. pylori to GPIbα expressed in CHO cells. CHO cells stably transfected with GPIbβ and GPIX were transfected transiently with GPIbα and gently mixed with H. pylori strain 60190. Mock transfected cells showed little binding of an (A ) anti-GPIb antibody and (B) did not bind H. pylori. Cells transfected with GPIbα showed increased binding of the (C ) anti-GPIb antibody and (D) supported binding of H. pylori. (E ) Adhesion of fluorescently labeled H. pylori was increased to CHO cells transfected with the GPIb complex and this was inhibited by AN51, an antibody to GPIbα Gastroenterology 2003 124, 1846-1854DOI: (10.1016/S0016-5085(03)00397-4)

Figure 4 The role of vWf in H. pylori-induced aggregation. (A ) Washed platelets failed to aggregate in response to H. pylori, even when supplemented with vWF, although an anti-vWf antibody inhibited aggregation in PRP. (B) The pro-aggregatory strain of H. pylori (60190) bound more vWf than the nonaggregatory strain J104 as measured by flow cytometry. Gastroenterology 2003 124, 1846-1854DOI: (10.1016/S0016-5085(03)00397-4)

Figure 5 The role of specific antibodies in H. pylori-induced platelet aggregation. The addition of pooled Ig and H. pylori to washed platelets caused aggregation to a similar extent as arachidonic acid. Pre-incubation of antibody with the bacteria followed by washing was equally effective. If the antibody was depleted by H. pylori first, aggregation was reduced, whereas depletion by S. sanguis had no effect. An antibody to FcγRIIA inhibited aggregation. Gastroenterology 2003 124, 1846-1854DOI: (10.1016/S0016-5085(03)00397-4)

Figure 6 Interaction between H. pylori and a platelet that leads to platelet activation. Gastroenterology 2003 124, 1846-1854DOI: (10.1016/S0016-5085(03)00397-4)