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Sandhya K. Balaram, MD, Devendra K. Agrawal, PhD, R

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1 Cell adhesion molecules and insulin-like growth factor-1 in vascular disease 
Sandhya K. Balaram, MD, Devendra K. Agrawal, PhD, R.Todd Allen, BA, Charles A. Kuszynski, PhD, John D. Edwards, MD  Journal of Vascular Surgery  Volume 25, Issue 5, Pages (May 1997) DOI: /S (97) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

2 Fig. 1 Dose-response effect of IGF-1 on monocyte adherence to HUVECs incubated with various doses of IGF-1 for 4 hours. Values are mean ± SEM. n = 7. *p < 0.01. Journal of Vascular Surgery  , DOI: ( /S (97) ) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

3 Fig. 2 Kinetics of IGF-1-induced increased monocyte adherence to HUVECs. Percent increase over baseline monocyte adherence (in absence of IGF-1) was calculated. Values are mean ± SEM. n = 4. *p < 0.01. Journal of Vascular Surgery  , DOI: ( /S (97) ) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

4 Fig. 3 Effect of IGF-1 in presence and absence of IGF-1 receptor antibody on monocyte adherence to HUVECs. HUVECs were incubated with IGF-1 receptor antibody (dilution 1:50) for 15 minutes before incubation with IGF-1 at 150 ng/ml. Values are mean ± SEM. n = 4. Journal of Vascular Surgery  , DOI: ( /S (97) ) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

5 Fig. 4 Effect of tyrosine kinase inhibitors on IGF-1-induced increased adherence to HUVECs. HUVECs were incubated with genistein (G) 100 μmol/L or herbimycin A (H) μmol/L for 15 minutes before the addition of IGF ng/ml. Values are mean ± SEM. n = 6. *p < 0.01. Journal of Vascular Surgery  , DOI: ( /S (97) ) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

6 Fig. 5 Time course of ICAM-1 expression of HUVECs treated with IGF-1 (150 ng/ml) as detected by immunoblotting with ICAM-1 antibody (dilution 1:1000). T, HUVECs treated with TNF-α (100 U/ml) for 4 hours used as a positive control. B, Baseline untreated HUVECs. Data are representative of three experiments. Journal of Vascular Surgery  , DOI: ( /S (97) ) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

7 Fig. 6 Time course of VCAM-1 expression of HUVECs treated with IGF-1 (150 ng/ml) as detected by immunoblotting with VCAM-1 antibody (dilution 1:1000). B, Baseline untreated HUVECs. Data are representative of three experiments. Journal of Vascular Surgery  , DOI: ( /S (97) ) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

8 Fig. 7 Analysis of surface expression of ICAM-1 (A), VCAM-1 (B), E-selectin (C), and P-selectin (D) on HUVECs incubated with IGF-1 (150 ng/ml) for 4 hours by flow cytometry. X axis represents fluorescence intensity, and Y axis represents relative number of cells. Journal of Vascular Surgery  , DOI: ( /S (97) ) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

9 Fig. 7 Analysis of surface expression of ICAM-1 (A), VCAM-1 (B), E-selectin (C), and P-selectin (D) on HUVECs incubated with IGF-1 (150 ng/ml) for 4 hours by flow cytometry. X axis represents fluorescence intensity, and Y axis represents relative number of cells. Journal of Vascular Surgery  , DOI: ( /S (97) ) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

10 Fig. 7 Analysis of surface expression of ICAM-1 (A), VCAM-1 (B), E-selectin (C), and P-selectin (D) on HUVECs incubated with IGF-1 (150 ng/ml) for 4 hours by flow cytometry. X axis represents fluorescence intensity, and Y axis represents relative number of cells. Journal of Vascular Surgery  , DOI: ( /S (97) ) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

11 Fig. 7 Analysis of surface expression of ICAM-1 (A), VCAM-1 (B), E-selectin (C), and P-selectin (D) on HUVECs incubated with IGF-1 (150 ng/ml) for 4 hours by flow cytometry. X axis represents fluorescence intensity, and Y axis represents relative number of cells. Journal of Vascular Surgery  , DOI: ( /S (97) ) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

12 Fig. 8 Mean fluorescence of ICAM-1 and VCAM-1 expression on HUVECs at various time periods after treatment with IGF-1 (150 ng/ml). Values are mean ± SEM. Data represent three experiments. *p < 0.05. Journal of Vascular Surgery  , DOI: ( /S (97) ) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

13 Fig. 9 Immunoreactivity of representative atherosclerotic aorta specimen stained with the following primary antibodies: Immunoglobulin G isotype control (A), von Willebrand's factor (B), ICAM-1 (C), VCAM-1 (D), E-selectin (E), and P-selectin (F). Specimens were then incubated in biotinylated secondary antibody and stained with immunoperoxidase. Journal of Vascular Surgery  , DOI: ( /S (97) ) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

14 Fig. 9 Immunoreactivity of representative atherosclerotic aorta specimen stained with the following primary antibodies: Immunoglobulin G isotype control (A), von Willebrand's factor (B), ICAM-1 (C), VCAM-1 (D), E-selectin (E), and P-selectin (F). Specimens were then incubated in biotinylated secondary antibody and stained with immunoperoxidase. Journal of Vascular Surgery  , DOI: ( /S (97) ) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

15 Fig. 9 Immunoreactivity of representative atherosclerotic aorta specimen stained with the following primary antibodies: Immunoglobulin G isotype control (A), von Willebrand's factor (B), ICAM-1 (C), VCAM-1 (D), E-selectin (E), and P-selectin (F). Specimens were then incubated in biotinylated secondary antibody and stained with immunoperoxidase. Journal of Vascular Surgery  , DOI: ( /S (97) ) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

16 Fig. 9 Immunoreactivity of representative atherosclerotic aorta specimen stained with the following primary antibodies: Immunoglobulin G isotype control (A), von Willebrand's factor (B), ICAM-1 (C), VCAM-1 (D), E-selectin (E), and P-selectin (F). Specimens were then incubated in biotinylated secondary antibody and stained with immunoperoxidase. Journal of Vascular Surgery  , DOI: ( /S (97) ) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

17 Fig. 9 Immunoreactivity of representative atherosclerotic aorta specimen stained with the following primary antibodies: Immunoglobulin G isotype control (A), von Willebrand's factor (B), ICAM-1 (C), VCAM-1 (D), E-selectin (E), and P-selectin (F). Specimens were then incubated in biotinylated secondary antibody and stained with immunoperoxidase. Journal of Vascular Surgery  , DOI: ( /S (97) ) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

18 Fig. 9 Immunoreactivity of representative atherosclerotic aorta specimen stained with the following primary antibodies: Immunoglobulin G isotype control (A), von Willebrand's factor (B), ICAM-1 (C), VCAM-1 (D), E-selectin (E), and P-selectin (F). Specimens were then incubated in biotinylated secondary antibody and stained with immunoperoxidase. Journal of Vascular Surgery  , DOI: ( /S (97) ) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

19 Fig. 10 Treatment with IGF-1 receptor primary antibody with secondary biotinylated antibody and peroxidase staining of a control aortic specimen (A) and an atherosclerotic aortic specimen (B). Journal of Vascular Surgery  , DOI: ( /S (97) ) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

20 Fig. 10 Treatment with IGF-1 receptor primary antibody with secondary biotinylated antibody and peroxidase staining of a control aortic specimen (A) and an atherosclerotic aortic specimen (B). Journal of Vascular Surgery  , DOI: ( /S (97) ) Copyright © 1997 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

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