Phylogenetic analysis of PurR from low-GC Gram-positive bacteria. Phylogenetic analysis of PurR from low-GC Gram-positive bacteria. (A) PurR sequences from representatives of the major bacterial lineages in the low-GC Gram-positive bacteria were aligned using the Clustal Omega program, and a phylogenetic tree was constructed using the ClustalW2 phylogeny program. (B) The type of PurBox used by each species was identified at the RegPrecise website (http://regprecise.lbl.gov/RegPrecise/collection_tffam.jsp?tffamily_id=53), and the two types of logo plots were constructed from the PurBox sequences presented for Streptococcaceae and Bacillales, respectively, using the weblogo service (http://weblogo.berkeley.edu/logo.cgi). Bjarne Hove-Jensen et al. Microbiol. Mol. Biol. Rev. 2017; doi:10.1128/MMBR.00040-16