Sequence similarity clusters of SET domain methyltransferases.

Slides:

Advertisements

Similar presentations

COLORING WITH NUMBERS. NumbersNumbers NumbersNumbers

Advertisements

Colors By Jes Betzold Red YellowBlue Orange PurpleGreen.

Monkey, Monkey In the Tree. Monkey, monkey in the tree Throw the yellow coconut down to me!

Guess the colour Mix the colours Evaluation Group with work.

©The Primary Art Class & Emily Gopaul

Sequence alignment of C-terminal phosphorylated plant aquaporins

NSAF and GeneChip datasets have similar PLGEM parameters.

Phosphorylation and sequence disorder in microtubule-associated protein Tau.A, schematic illustration of the domain profile of Tau with all known phosphorylation.

Distribution of phosphorylation sites identified in the cytosolic phosphoproteome.A, numbers of approved phosphopeptides, previously phosphorylated peptides,

Classification of proteins in PC-3 cell-released microvesicles by GOslim annotations. Classification of proteins in PC-3 cell-released microvesicles by.

Chapter 26 Proteomics: The Global Analysis of Proteins

What Color is it?.

Percentage of proteins identified in envelope membrane extracts according to the purification method and the number of transmembrane domains. Percentage.

C c Cc is for cat. © ©

Frequency distribution of the GRAVY of the theoretical proteins (open bars) and of 110 genes encoding proteins identified on a 2-D electrophoresis gel,

Top-down protein identification.

Distributions of the ELDP values and Mascot scores for all protein identifications.a, frequency of ELDP value returned by correct (gray bars) and incorrect.

Sequence similarity cluster of SPOUT methyltransferases.

Significantly enriched phosphorylation motifs from up-regulated phosphopeptides by Motif-X analysis. Significantly enriched phosphorylation motifs from.

Epitope Mapping Performance using a single peptide microarray.

Global visualization of antigen and epitope discovery.

A, Averaged full MS (ions converted to monoisotopic MW by Xcalibur Xtract) of Segment I-3 (see supplemental Fig. A, Averaged full MS (ions converted to.

Bottom-up proteomic characterization of MALDI IMS samples.

Mass spectrometry identification of spots in 2D blue native gels of cytoplasmic membranes isolated from BL21(DE3)pLysS Spots in 2D BN gels of cytoplasmic.

Technical reproducibility and biological variability.

Correction of translational start site by identification of N-terminal peptide. Correction of translational start site by identification of N-terminal.

A, myelinated nerve fibers in the control and biopsied mouse striatum were stained with anti-MBP antibody. A, myelinated nerve fibers in the control and.

The evolutionary conservation of the phosphoproteomes.a, E. coli. b, B. subtilis. The evolutionary conservation of the phosphoproteomes.a, E. coli. b,

Cluster analysis and pathway-based characterization of differentially expressed genes and proteins from integrated proteomics. Cluster analysis and pathway-based.

Colonopshere-enriched proteins display functional interactions.

Proteins previously reported in published MALDI IMS studies and their frequency of observation in the present study. Proteins previously reported in published.

Confocal fluorescence microscopical images of 3-nitrotyrosine-positive blood vessels from a patient with mitochondrial disease. Confocal fluorescence microscopical.

Homologs between yeast and human seven-β-strand methyltransferases.

High correlation of expression changes of NMD-regulated genes identified by both the pSILAC screen and previously reported global RNA screens after UPF1.

N-terminal extension of a gene using peptides mapping upstream to an annotated start site. N-terminal extension of a gene using peptides mapping upstream.

Bioinformatics approaches for searching proteome for methyltransferase family members. Bioinformatics approaches for searching proteome for methyltransferase.

Protein microarrays for validation of antibodies.

MS/MS spectra of INEILSNALKR with a Lys residue modified with SUMO1 or SUMO3 remnant chains. MS/MS spectra of INEILSNALKR with a Lys residue modified with.

Two-dimensional gel electrophoresis of CyDye-labeled human sperm proteomes (DIGE) and identification of diabetes- and obesity-associated sperm proteins.

Testing the effectiveness of the three-step peptide fractionation method.A, μLC mass chromatograms of SCX fractions for an acidic FFE fraction. Testing.

Resolution and mass accuracy of A, a peptide isotope cluster (m/z 558

Putative targets of miRNAs directly induced by p53 with down-regulated mRNA- and de novo protein synthesis or reduced de novo protein synthesis only. Putative.

Interaction networks of the regulated phosphoproteins.

Identification of chaperonin GroEL (Rv0440) with representative MS/MS spectrum. Identification of chaperonin GroEL (Rv0440) with representative MS/MS spectrum.A,

Distribution of the phosphoproteins based on GO analysis, including biological process (Left) and cellular component (Right). Distribution of the phosphoproteins.

A, Absolute ion intensities of m/z 322, 922 and 1522 as function of the transfer time. A, Absolute ion intensities of m/z 322, 922 and 1522 as function.

K-Means clustering of protein and mRNA expression patterns after PPAR agonists treatments. k-Means clustering of protein and mRNA expression patterns after.

Comparison of mapped epitopes and peptides identified in immuno-SILAC screening of polyclonal antibodies against trypsin-digested PrESTs. Comparison of.

Number of genes/antibodies included in the database.

The fluorescent receptor chains exhibit their characteristic fluorescent signatures: images and spectra of human IFN-γR2/GFP and IFN-γR2/EBFP transfected.

Immuno-MS results from antibodies toward 20 different target proteins in HeLa cell lysates. Immuno-MS results from antibodies toward 20 different target.

Bar plot representation of the transcriptomic changes in Δsaci_ptp and Δsaci_pp2a. Bar plot representation of the transcriptomic changes in Δsaci_ptp and.

Localization of selected clones in mammalian COS-7 cells.

Analytical metrics of yeast proteome analysis using the Q-OT-qIT (Fusion) as compared with qIT-OT (Orbitrap Elite) and Q-OT (Q-Exactive) hybrids. Analytical.

Biochemical characterization of the protein phosphatases Saci-PTP.

Voronoi treemaps (109) comparing protein expression profiles of M

A simplified example of a protein summary list.

Classification of the 1458 identified proteins into molecular functions. Classification of the 1458 identified proteins into molecular functions. The pie.

Separation of colonospheres from differentiated tumor cells by cluster analysis. Separation of colonospheres from differentiated tumor cells by cluster.

2-D gel images visualized by Coomassie Brilliant Blue staining representing total proteins extracted from HCT-8 under apoptotic conditions in 2 mm Gln.

Schematic of AIMS-to-MRM experiment.

GeneGoTM-based signaling pathway annotations of proteins identified in CD56+ NK cell subsets. GeneGoTM-based signaling pathway annotations of proteins.

Changes in protein expression during distinct stages of NK cell differentiation. Changes in protein expression during distinct stages of NK cell differentiation.

Differential protein, mRNA, lncRNA and miRNA regulation by p53.

Model of the change in receptor structure on engagement of the ligand IFN-γ. Model of the change in receptor structure on engagement of the ligand IFN-γ.

Presentation transcript:

Sequence similarity clusters of SET domain methyltransferases. Sequence similarity clusters of SET domain methyltransferases. All of the known and putative SET domain methyltransferases were analyzed by CLANS with reference proteins to infer substrate specificity. All human SET domain methyltransferases are in black. Reference proteins methylating only H3K4 are in red; those methylating H3K9 are in yellow; those methylating H3K36 are in blue; those methylating both H3K36 and H4K20 are in green; those methylating H3K9, H3K27, and H4K20 are in pink; and those methylating Rubisco are in purple. BLAST correlations are shown with gray lines; lighter shades of gray represent BLAST correlations closer to an E-value of 1, whereas darker shades of gray represent BLAST correlations that are closer to an E-value of 0. Tanya C. Petrossian, and Steven G. Clarke Mol Cell Proteomics 2011;10:M110.000976 © 2011 by The American Society for Biochemistry and Molecular Biology, Inc.