Opening Activity: March 20, 2017 In your opening activity write today’s goals: Take notes in journal on polymorphisms and banding patterns (Sub will show you 2 slides) Complete Elephant DNA Analysis (due today) Read through Lab #17, tape into journal. Create a picture of tubes 1-6 and draw what is going into each tube. Example on board. Work on Manipulating DNA reading OR Idea Journal on back of Elephant DNA Analysis. Homework: Manipulating DNA Reading 3/24 Test Retake by 3/24 I can… Explain how biotech tools are used to analyze DNA.

How do scientists compare DNA? Polymorphisms – Differences in DNA sequence duce to mutations or changes n the sequence.

How do scientists compare DNA? Banding Patterns – A pattern visible on a gel after DNA gel electrophoresis. The pattern is unique to an individual or populations of organisms because they share similar DNA. Example of banding Patterns used in to solve a Crime, blood stain and 4 suspects. Who did it?

Opening Activity: March 21, 2017 Turn in “Elephant DNA Analysis” from yesterday. What two actions will you complete today as to prepare to run our gel electrophoresis? Where will you keep your sample solutions today as you work today? When you are measuring out 3µl, which pipet will you use? I can prepare…. A restriction digest for the Ivory sample. DNA samples from Africa to run gel. Homework: Manipulating DNA Reading 3/24 Test Retake by 3/24

Today’s Goals Create a restriction digest for the ivory sample Accurately measure out your control tubes for running on gel for Thursday. Bring tubes to center cart to go into freezer overnight. Put tube #6 in hot water bath. Clean up!! (everything put away and wiped down for 5 pts today)

Prepare Tubes (25 min) Keep all samples on ice when not in use. Label 6 tubes with period, table number, and tube number. As you prepare tube, CHECK OFF each item to be sure all reagents are put in. Prepare tube #6, restriction digest first. Put tube #6 in incubator for 30 minutes. Prepare tubes #1-#5. All tubes to center cart to be put in freezer.

What did we do today? Explain a restriction digest, what reagents go into a microtube to create the digest. Explain why we did a digest today. Explain the purpose of the tubes 3, 4 & 5. Why will it be important to run these samples on a gel? What is a DNA ladder, what purpose will the ladder serve on our gel? Why did we prepare a tube of uncut DNA, explain the importance of this tube and what information it will tell us on a gel.

What did we do today? Explain a restriction digest, what reagents go into a microtube to create the digest. Explain why we did a digest today. Explain the purpose of the tubes 3, 4 & 5. Why will it be important to run these samples on a gel?

What did we do today? What is a DNA ladder, what purpose will the ladder serve on our gel? Why did we prepare a tube of uncut DNA, explain the importance of this tube and what information it will tell us on a gel.

Opening Activity: March 22, 2017 Read Lab #17 Day 2. What will be accomplished today? How much agarose will you measure out and pour into the gel box? I can… Practice making a gel and loading sample dyes into the gel. Homework: Manipulating DNA Reading 3/24 Test Retake by 3/24 Micropipet Quiz Corrections due 3/24

Today’s Goals Create an agarose gel in your gel electrophoresis. Practice loading gels with practice dye. Clean up!! (everything put away and wiped down for 5 pts today) Complete Genetic Manipulation Reading

Pouring Gel (5-10 minutes) Put your dams and comb in correctly, remember DNA is negatively charged so it will run to the positive end. Measure out 25ml of agarose from hot water bath - CAUTION HOT!! Use GLOVES. Gently pour agarose SLOWLY between the dams. LET STAND FOR 10 MINUTES. While you wait…. Complete Genetic Manipulating Reading and Questions.

Practice Loading Gel (10 min) Remove dams and comb. Measure out and pour 125 ml of water over gel into gel box, about 1ml over gel. Use dye sample to load 10 µl into each well. Let everyone practice loading the gel. Clean up – water down sink, agarose in garbage.

Opening Activity: March 23, 2017 Read Lab #17 Day 3. What will be accomplished today? Instead of water today over our gel, what will we use? How much loading dye will we add to each tube? Homework: Manipulating DNA Reading 3/24 Test Retake by 3/24 Micropipet Quiz Corrections due 3/24 I can… Load gel with DNA and run for 45 minutes.

Today’s Goals Create an agarose gel in your gel electrophoresis. Add Sample Loading Buffer to all tubes. Add 125ml of Running Buffer to gel box. Arrange gel box in a sturdy position near power and load gels, 15µl to each well. Create a picture of your gel box to be sure you know what was loaded into each well. Clean up!! Timekeeper?

Opening Activity: March 24, 2017 Pick up African Map, Video Questions and Elephant DNA analysis at front table – tape in. Review your DNA Analysis: Review how the restriction enzyme cut, did your cut between the two Gs? On top and bottom? Review how to count a fragment size, did you count ONLY the connected base pairs? Which fragments on your gel won? Long or short? I can… Explain polymorphisms and how it causes different fragment sizes of DNA. Homework: DNA Reading 3/24 Test Retake by 3/24 Micropipet Quiz Corrections due 3/24