1. Feb 3, 2015 I can… Predict how DNA is used to solve real- world problems Reminders: February 4 th – All first semester work must be turned in, please check the source! 1.DNA is a biomolecule, what atoms do you predict DNA is made from? 2.What happens during interphase of the cell cycle that prepares the cell for mitosis?

2. Feb 4, 2015 I can… Construct a DNA model and identify the 4 nucleotides that make up DNA. Reminders: All first semester work must be turned in TODAY! 1.Pick up reading guide at r. table – read, highlight, underline, etc. A.What does DNA stand for? B.What is the name of the sugar molecule in the DNA molecule? C. What is complimentary base pairing? D. How are genes related to DNA?

3. Feb 5, 2015 I can… Identify steps in DNA replication to ensure an exact copy is prepared for the daughter cells.. Reminders: All first semester work must be turned in TODAY – LAST CHANCE!!!! Quiz – Fri 2/13 (DNA, Replication & Pipets) 1. Suppose you know that the sequence on one strand of DNA is AGCTCAG. What is the sequence of the bases on the opposite strand? 2.What are the subunits of DNA? 3.Missing a 12 th night book? Black fleece?

4. Feb 6, 2015 I can… Use a micropipet, accurately measure out small amounts of liquid. Reminders: Quiz – Fri 2/13 (DNA, Replication & Pipets) 1.Pick up micropipet notes at resource table tape in. I will stamp your coloring and questions. 2.Assume a 100-base pair DNA molecule contains 45 cytosine. How many adenines are there? 2.What molecule helps “unzip” DNA for replication? 3.Where do you think DNA replication takes place for a bacteria cell?

5. Feb 9, 2015 I can… Measure amounts or liquid accurately with a micropipet and use a centrifuge. Reminders: Quiz – Fri 2/13 (DNA, Replication & Pipets) HW Reading Questions Opening Activity: What is a micropipette? Which pipette would pick up: 8 µl, 32 µl, 155 µl? 1 ml = __________ µl

6. Feb 10, 2015 I can… Explain how DNA can be manipulated by restriction enzymes. Homework: DNA, DNA Replication and Micropipette Quiz 2/13 Vocabulary sheet due 2/13 STAY CAUGHT UP!! 1.I will stamp reading “Landmark Discovery…” 2.What do we mean by a balanced centrifuge? 3.What are the steps to release liquid from your pipette? 4.What is a restriction enzyme?

7. Feb 11, 2015 I can… Create a recombinant DNA. Homework: DNA, DNA Replication and Micropipette Quiz 2/13 Vocabulary sheet due 2/13 STAY CAUGHT UP!! Opening Activity: 1.I will stamp restriction enzyme practice. 2.How do restriction enzymes help with manipulating DNA? How do they work? 3. If a strand of DNA was recognized by EcoRI 3 times, what would the final DNA look like?

8. Feb 12, 2015 I can… Explain the evolution of DNA manipulation. Homework: DNA, DNA Replication and Micropipette Quiz 2/13 Vocabulary sheet due 2/13 STAY CAUGHT UP!! Opening Activity: 1.Pick up reading at resource table, Manipulating DNA, tape in. 2.Define the following: plasmid, restriction site, recombinant DNA.

9. Feb 13, 2015 I can… Share my knowledge of DNA, DNA Replication and micropipettes. Explain gel electrophoresis Homework: Manipulating Genetic Material stamped 2/18 Look at class website or Sample NB to stay caught up! Opening Activity: 1.Have your DNA vocab sheet available, Ms. Erb will come around and stamp. 2.Be sure to check class website, sample notebook, table of contents and the file folders if you were gone. Please be caught up when you return on Wednesday.

10. Feb 18, 2015 I can… Explain gel electrophoresis and demonstrate a model sample “run” of DNA. Homework: Biotech QUEST 3/4 Welcome back! 1.I will stamp your “DNA Goes to the Races Reading”, if you did not finish last week, finish now. 2.What is a gel electrophoresis?

11. Feb 19, 2015 I can… Load a gel to study DNA. Perform a practice digest with elephant DNA. Homework: Biotech Test March 4th Elephant DNA Practice Opening Activity: 1.Find “Practice Gel Formation and Loading of Gel”, READ. Set up your Gel box so you are prepared to pour a gel. 2.How much agarose will you measure out and pour into the gel box? 3.How long will it take for the gel to “set”?

12. Feb 20, 2015 I can… Prepare DNA to study on a gel. Digest the ivory DNA with restriction enzymes. Homework: Biotech Test March 4th 1.I will stamp your Elephant DNA practice. 2.Pick up Lab #17, tape in and READ. 3.What solutions will you put into your tube to perform the digest of the ivory DNA? 4.When you are measuring out 3µl, which pipet will you use?

13. Feb. 23, 2015 I can… Load a gel with DNA to study the banding patterns from elephants and discover where origin of the ivory. Homework: Biotech TEST 3/4 Opening Activity: 1.Read Part #2 from Elephant Lab in journal… a.What will we use today to cover the gel, instead of water? b.What will you add to each tube today? c.At what voltage will our DNA run today?

14. Feb 24, 2015 I can… Explain polymorphisms and how it causes different fragment sizes of DNA. Describe where E. DNA was collected. Homework: Biotech TEST 3/4 Opening Activity: 1.Pick up two items at Resource Table to tape into journal: Map of Africa Elephant Video Questions

15. Feb. 25, 2015 I can… Interpret elephant DNA banding patterns from our gels and determine where origin of the ivory. Homework: Biotech TEST 3/4 Review Sheet 3/4 Lab #17 Lab Report 2/27 Opening Activity: 1.Pick up the following items at the resource table a.Biotechnology Review Sheet – TAPE IN b.Orange/Yellow Data Submission Form 2.What is a banding pattern and how will we use them to determine the origin of our confiscated ivory?

16. Feb 26, 2015 I can… Interpret elephant DNA banding patterns from our gels and determine where origin of the ivory. Homework: Biotech TEST 3/4 Review Sheet 3/4 Lab #17 Lab Report 2/27 Opening Activity: 1.Pick up Lab #17 Elephant DNA Rubric - READ 2.What will be included in your claim for your lab report? 3.What is the scientific explanation for why two elephants different banding patterns? Why would two elephants have the same banding patterns?

17. March 2, 2015 I can… Explain sustainability and practice application EOC questions. Homework: Biotech TEST 3/4 Review Sheet 3/4 Lab #17 Lab Report Tues Opening Activity: 1.Turn in Lab #17 report to basket, if completed. Are all the lanes labeled? 2.Pick up Sustainability and Application packet at resource table.

18. March 3, 2015 I can… practice application EOC questions. Homework: Biotech TEST 3/4 Review Sheet 3/4 Lab #17 Lab Report Today 1.Turn in Lab #17 report to basket, due today! Are all the lanes labeled? 2.Find your EOC Practice Handout. I will stamp practice problem #1. 3.Continue working on ALL the practice problems, INDIVIDUALLY!