Alan P Knutsen, Clifford Bellone, Henk Kauffman

Slides:

Advertisements

Similar presentations

Impaired TH17 responses in patients with chronic mucocutaneous candidiasis with and without autoimmune polyendocrinopathy–candidiasis–ectodermal dystrophy

Advertisements

Cost of care and clinical condition in paediatric cystic fibrosis patients Ulrich Baumann, Christiane Stocklossa, Wolfgang Greiner, Johann-Matthias Graf.

Volume 27, Issue 4, Pages (October 2007)

The epidemiology of the atopic child

by Masih Ostad, Margareta Andersson, Astrid Gruber, and Anne Sundblad

Monocyte chemoattractant chemokines in cystic fibrosis

Reduced levels of active GLP-1 in patients with cystic fibrosis with and without diabetes mellitus Magnus Hillman, Leif Eriksson, Lena Mared, Karin Helgesson,

Impaired TH17 responses in patients with chronic mucocutaneous candidiasis with and without autoimmune polyendocrinopathy–candidiasis–ectodermal dystrophy

Reduced IFN-γ receptor expression and attenuated IFN-γ response by dendritic cells in patients with atopic dermatitis Eva Gros, MSc, Susanne Petzold,

A novel mutation in CD132 causes X-CID with defective T-cell activation and impaired humoral reactivity Taco W. Kuijpers, MD, PhD, Paul A. Baars, PhD,

Differential cytokine induction by the human skin–associated autoallergen thioredoxin in sensitized patients with atopic dermatitis and healthy control.

Predominance of type 2 cytokine–producing CD4+ and CD8+ cells in patients with atopic dermatitis Masatoshi Nakazawa, DVMa, Nakako Sugi, MDb, Hiroshi.

Blood basophils from cystic fibrosis patients with allergic bronchopulmonary aspergillosis are primed and hyper-responsive to stimulation by aspergillus.

Volume 26, Issue 3, Pages (March 2007)

Impaired negative regulation of homeostatically proliferating T cells

Cytokine and chemokine expression of flucloxacillin-specific T cell clones (TCC) of patient P1. Cytokine and chemokine expression of flucloxacillin-specific.

Enhancement of the host immune responses in cutaneous T-cell lymphoma by CpG oligodeoxynucleotides and IL-15 by Maria Wysocka, Bernice M. Benoit, Sarah.

Anna L. P. Chapman, Brian M. Morrissey, Vihas T. Vasu, Maya M

IL-4 confers resistance to IL-27–mediated suppression on CD4+ T cells by impairing signal transducer and activator of transcription 1 signaling Zhihong.

Efalizumab Therapy for Atopic Dermatitis Causes Marked Increases in Circulating Effector Memory CD4+ T Cells That Express Cutaneous Lymphocyte Antigen

Differential control of TH1 versus TH2 cell responses by the combination of low-dose steroids with β2-adrenergic agonists Elena Goleva, PhD, Annegret.

IL-13-Stimulated Human Keratinocytes Preferentially Attract CD4+CCR4+ T cells: Possible Role in Atopic Dermatitis Rahul Purwar, Thomas Werfel, Miriam.

IL-10 inhibits CD28 and ICOS costimulations of T cells via src homology 2 domain— containing protein tyrosine phosphatase 1 Alison Taylor, PhD, Mübeccel.

Human osteoarthritic chondrocytes are impaired in matrix metalloproteinase-13 inhibition by IFN-γ due to reduced IFN-γ receptor levels R. Ahmad, M. El.

FR255734, a Humanized, Fc-Silent, Anti-CD28 Antibody, Improves Psoriasis in the SCID Mouse-Psoriasis Xenograft Model Siba P. Raychaudhuri, Smriti Kundu-Raychaudhuri,

Pseudomonas aeruginosa in cystic fibrosis: Pyocyanin negative strains are associated with BPI-ANCA and progressive lung disease Malin Carlsson, Swati.

Volume 124, Issue 3, Pages (March 2003)

Age-Related Differences in Sensitivity of Peripheral Blood Monocytes to Lipopolysaccharide and Staphylococcus Aureus Toxin B in Atopic Dermatitis Marie.

Kathleen R. Bartemes, BA, Gail M. Kephart, BS, Stephanie J

Increased serum concentration of G-CSF in cystic fibrosis patients with chronic Pseudomonas aeruginosa pneumonia P.Ø. Jensen, C. Moser, A. Kharazmi,

Bronchial epithelial cell lines and primary nasal epithelial cells from cystic fibrosis respond differently to cigarette smoke exposure Mark Thomas Shaw.

Katherine G. MacDonald, BSc, Nicholas A. J

(A) Western ... (A) Western blot showing DTX1 knockdown with siRNA in human CD4+ T cells. Actin was used as the loading control. (B) Purified.

Atul Sathe, Sterling B. Ortega, Dorothy I. Mundy, Robert H

T Cell Epitope-Specific Defects in the Immune Response to Cat Allergen in Patients with Atopic Dermatitis Raquel Carneiro, Amanda Reefer, Barbara Wilson,

Volume 39, Issue 4, Pages (October 2003)

β-Agonist enhances type 2 T-cell survival and accumulation

Peanut-specific type 1 regulatory T cells induced in vitro from allergic subjects are functionally impaired Laurence Pellerin, PhD, Jennifer Anne Jenks,

Different opinions of physicians on the importance of measures to prevent acquisition of Pseudomonas aeruginosa from the environment Gratiana Steinkamp,

Reciprocal Regulation of Thymus and Activation-Regulated Chemokine/Macrophage- Derived Chemokine Production by Interleukin (IL)-4/IL-13 and Interferon-γ.

The relative contribution of IL-4 and IL-13 to human IgE synthesis induced by activated CD4+ or CD8+ T cells Juha Punnonen, MD, PhD, Hans Yssel, PhD,

Increased expression of Interleukin-13 but not Interleukin-4 in cystic fibrosis patients Hans-Peter Hauber, Djalal Gholami, Gerhard Koppermann, Hans-Eberhard.

D.Y.F. Mak, J. Sykes, A.L. Stephenson, L.C. Lands

CX-659S, a Diaminouracil Derivative, Indirectly Inhibits the Function of Langerhans Cells by Blocking the MEK1/2-Erk1/2 Pathway in Keratinocytes Hiroshi.

Peanut T-cell epitope discovery: Ara h 1

Cell surface characterization of T lymphocytes and allergen-specific T cell clones: Correlation of CD26 expression with T H1 subsets Martin Willheim,

IL-12 affects Dermatophagoides farinae–induced IL-4 production by T cells from pediatric patients with mite-sensitive asthma Takeshi Noma, MD, PhD, Izumi.

A.K.I. Kuroki, Masayuki Iyoda, Takanori Shibata, Tetsuzo Sugisaki

Interferon-γ, a Strong Suppressor of Cell Proliferation, Induces Upregulation of Keratin K6, One of the Inflammatory- and Proliferation-Associated Keratins

Johannes Hampl, Yueh-hsiu Chien, Mark M Davis Immunity

Increased expression of the CD80 accessory molecule by alveolar macrophages in asthmatic subjects and its functional involvement in allergen presentation.

Decreased interleukin-18 expression in BAL cells and peripheral blood mononuclear cells in adult cystic fibrosis patients Hans-Peter Hauber, Inga S Beyer,

High-dimensional analysis of effector CD4 T cell function following γHV68 infection in B6 and IL-10KO mice. High-dimensional analysis of effector CD4 T.

Matthew J. Loza, PhD, Susan Foster, PhD, Stephen P

Human γ/δ T-cell proliferation and IFN-γ production induced by hexamethylene diisocyanate Adam V Wisnewski, PhD, Christina A Herrick, MD, PhD, Qing Liu,

N Dauletbaev, K Viel, R Buhl, T.O.F Wagner, J Bargon

Harald Renz, MD, Chaya Brodie, PhD, Katherine Bradley, BS, Donald Y. M

Allergic rhinitis Journal of Allergy and Clinical Immunology

Food allergen (peanut)–specific T H2 clones generated from the peripheral blood of a patient with peanut allergy Esther C. de Jong, MSca, b, Steven Spanhaak,

Volume 57, Issue 2, Pages (October 2000)

IL-10–producing monocytes differentiate to alternatively activated macrophages and are increased in atopic patients Antje Prasse, MD, Martin Germann,

Mechanisms of inhibition of IgE synthesis by nedocromil sodium: Nedocromil sodium inhibits deletional switch recombination in human B cells Richard K.S.

The novel synthetic immune response modifier R-848 (Resiquimod) shifts human allergen-specific CD4+ TH2 lymphocytes into IFN-γ–producing cells Francesca.

Possible Pathogenic Role of Th17 Cells for Atopic Dermatitis

Increased Type 2 Cytokine Expression by Both CD4+ CD45RO+ T Cells and CD8+ CD45RO+ T Cells in Blood Circulation is Associated with High Serum IgE but.

Volume 126, Issue 5, Pages (May 2004)

Distributions of CD4, Th1, and Th2 in adults (n = 16; A) and children (n = 50; B). Distributions of CD4, Th1, and Th2 in adults (n = 16; A) and children.

Volume 8, Issue 5, Pages (May 1998)

(A) Western ... (A) Western blot showing DTX1 knockdown with siRNA in human CD4+ T cells. Actin was used as the loading control. (B) Purified.

Thymocyte Glucocorticoid Resistance Alters Positive Selection and Inhibits Autoimmunity and Lymphoproliferative Disease in MRL-lpr/lprMice Eva Tolosa,

Presentation transcript:

Immunopathogenesis of allergic bronchopulmonary aspergillosis in cystic fibrosis Alan P Knutsen, Clifford Bellone, Henk Kauffman Journal of Cystic Fibrosis Volume 1, Issue 2, Pages 76-89 (June 2002) DOI: 10.1016/S1569-1993(02)00033-4

Fig. 1 Model of pathogenesis of allergic bronchopulmonary aspergillosis (from Knutsen et al. [1]). Journal of Cystic Fibrosis 2002 1, 76-89DOI: (10.1016/S1569-1993(02)00033-4)

Fig. 2 Asp f1-specific T-cell line was rested for 2–17 days after IL-2 and Asp f1 stimulation (top panel). Supernatant IL-4 concentration was measured serially. Significant amounts of IL-4 were synthesized for up to 10–14 days of rest. Monoclonal anti-IL-4 antibody inhibited Asp f1-stimulated lymphoproliferation of antigen-specific T-cell lines in a dose–response manner (bottom panel). Maximum inhibition was 84% at 5% anti-IL-4 antibody concentration (v/v) in this experiment and 78% in a second experiment. Anti-IL-2 had little inhibition of proliferation, indicating an IL-4-dependent autocrine lymphoproliferative response (from Knutsen et al. [34]). Journal of Cystic Fibrosis 2002 1, 76-89DOI: (10.1016/S1569-1993(02)00033-4)

Fig. 3 IL-4 induction of CD23+ molecules on CD20+ and on CD86+ CD20+ B-cells. ABPA CF patients had significantly increased number of CD23 molecules (a) per B-cell and (b) per CD86+ B-cell on day 0 compared to non-ABPA CF patients (P<0.01) and non-atopic control patients (P<0.01). Following IL-4 stimulation, ABPA CF patients had a significantly increased rate of CD23+ expression (a) per B-cell and (b) per CD86+ B-cell compared to non-ABPA CF (P<0.01) and non-atopic control patients (P<0.01). Data presented as mean±S.E. (from Knutsen et al. [66]). Journal of Cystic Fibrosis 2002 1, 76-89DOI: (10.1016/S1569-1993(02)00033-4)

Fig. 4 IL-13 induction of CD23+ molecules on CD20+ and on CD86+ CD20+ B-cells. Following IL-13 stimulation, ABPA CF patients had no significantly increased rate of CD23+ expression (a) per B-cell and (b0 per CD86+ B-cell compared to non-ABPA CF and non-atopic control patients (P<0.01). Data presented as mean±S.E. (from Knutsen et al. [66]). Journal of Cystic Fibrosis 2002 1, 76-89DOI: (10.1016/S1569-1993(02)00033-4)

Fig. 5 Th1 and Th2 CD3+ T cells. (a) Following PMA and ionomycin stimulation, ABPA CF patients had comparable percentages of IFN-γ and IL-4 CD3+ T-cells compared to non-ABPA CF patients. However, both ABPA and non-ABPA CF patients had significantly decreased percentages of IFN-γ CD3+ T-cells compared to normal control subjects (P<0.001, P<0.001 respectively). (b) Following tetanus toxoid and Asp f2/3/4 stimulation, both ABPA and non-ABPA CF patients had significantly decreased IFN-γ CD3+ T-cells compared to normal control subjects (P<0.05, P<0.05, respectively) but not different from each other. However, ABPA CF patients had significantly increased Asp f2/3/4-stimulated IL-4 CD3+ T-cells compared to non-ABPA CF patients and normal control subjects (P<0.01, P<0.01, respectively). Data presented as mean±S.E. (from Knutsen et al. [66]). Journal of Cystic Fibrosis 2002 1, 76-89DOI: (10.1016/S1569-1993(02)00033-4)