1. Possible Pathogenic Role of Th17 Cells for Atopic Dermatitis
Chizuko Koga, Kenji Kabashima, Noriko Shiraishi, Miwa Kobayashi, Yoshiki Tokura 
Journal of Investigative Dermatology 
Volume 128, Issue 11, Pages (November 2008)
DOI: /jid
Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

2. Figure 1
Lactate dehydrogenase levels in different severity groups of AD patients. The AD patients were classified as acute (n=23), mild (n=7), moderate (n=5), and severe (n=11) by clinical findings, and serum levels of lactate dehydrogenase from AD patients were compared with that in healthy donors. Student's t-test was performed between the indicated groups and an asterisk indicates P< 0.05.
Journal of Investigative Dermatology  , DOI: ( /jid )
Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

3. Figure 2
Flow cytometric analysis of IL-17+ cells in peripheral blood lymphocytes. PBMCs isolated from a 30-year-old man with AD were stimulated with PMA and ionomycin for 8hours and Golgistop was added. Then, production of IL-17 by PBMCs was determined at the single-cell level by intracellular cytokine staining and flow cytometric analysis. The numbers in the upper and lower right quadrants represent the percentage of IL-17+ cells with or without expression of CD3+ or CD8+ cells in the total lymphocyte populations gated.
Journal of Investigative Dermatology  , DOI: ( /jid )
Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

4. Figure 3
Percentage of circulating IL-17+ T cells in relation to AD severity and IFN-γ+ T cells. AD patients were divided into three different severity groups (mild, n=7; moderate, n=5; and severe, n=11). (a) Healthy subjects (n=9), three groups of AD, psoriatic patients (n=16) were compared for percentage of IL-17+ and CD4+ or CD8+ T cells, and a significant correlation between healthy donors and severe AD patients was found (P=0.03). (b) IFN-γ+ T cells of all AD patients were also analyzed. A significant correlation between percentage of IL-17+ CD4+ T cells and IFN-γ+ T cells was found (P=0.0009). In addition, the correlation (P-value) between the percentage of IL-17+ CD4+ T cells and IFN-γ+ T cells among acute, moderate, and severe AD patients was depicted.
Journal of Investigative Dermatology  , DOI: ( /jid )
Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

5. Figure 4
Immunohistochemistry of IL-17+ cells in skin lesions of AD. (a) A skin specimen from an AD patient was immunohistochemically stained for IL-17. Left, low-magnification image of acute lesion; top right, high-magnification image of acute lesion; and bottom right, high-magnification image of chronic lesion. Bar=30μm. (b) The number of IL-17+ cells was enumerated in acute and chronic lesions from mild, moderate, and severe cases of AD. Columns show mean±SD. Student's t-test was performed between the indicated groups and an the asterisk indicates P<0.05.
Journal of Investigative Dermatology  , DOI: ( /jid )
Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

6. Figure 5
Effects of IL-17 and/or IL-22 on keratinocyte production of cytokines/chemokines and VEGF. NHEKs were cultured with or without IL-17 and/or IL-22 at the indicated doses. Three-day culture supernatants were subjected to analysis with the cytometric beads array system or ELISA. Columns show mean±SD of data using triplicated wells. The asterisk indicates statistically significant differences compared with the untreated group (P<0.05, unpaired two-tailed t-test). Data are representative of those from three independent experiments.
Journal of Investigative Dermatology  , DOI: ( /jid )
Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions