Supplementary Figure 4. Comparisons of MethyLight and gene expression data. PMR values (X-axis) were plotted against log2 gene expression values (Y-axis)

Slides:



Advertisements
Similar presentations
BME 130 – Genomes Lecture 7 Genome Annotation I – Gene finding & function predictions.
Advertisements

Supplementary Material Supplementary Tables Supplementary Table 1. Sequencing statistics for ChIP-seq samples. Supplementary Table 2. Pearson correlation.
Supplementary Material Epigenetic histone modifications of human transposable elements: genome defense versus exaptation Ahsan Huda, Leonardo Mariño-Ramírez.
Supplementary file 3 Concentration of the plasmid containing a single copy of MusaSAP1 5’ UTR : 50 ng/μl Total length of the plasmid containing a single.
COURSE OF BIOINFORMATICS Exam_31/01/2014 A.
Supplementary Figure S1 Percentage of peaks from Trf1 +/+ p53 -/- -Cre vs Trf1  /  p53 -/- -Cre comparison that are located in non subtelomeric and subtelomeric.
DNA Organization, Replication, & Repair. Model for the structure of the nucleosome.
Supplemental Figure 1A. A small fraction of genes were mapped to >=20 SNPs. Supplemental Figure 1B. The density of distance from the position of an associated.
Supplementary Figure 1 Gene A1 st Gene B1 st Gene C1 st ~ Gene G1 st 2 nd ~ 19 th Gene H1 st 2 nd ~ 19 th Gene I1 st 2 nd ~ 19 th ~ 1 st 2 nd 19 th Gene.
A B IL-4(+) IL-4(-) IL-4(+) IL-4(-) ChIP-Seq (STAT6) Ramos IL-4 (+) P-value Ramos IL-4 (-) P-value BEAS2B IL-4 (+) P-value BEASB IL-4 (-) P-value fold.
5.4 Line of Best Fit Given the following scatter plots, draw in your line of best fit and classify the type of relationship: Strong Positive Linear Strong.
Figure S1 Enod11 Mtc27 MtGshs cDNA gDNA S. meliloti DPI Supporting Information Fig. S1. Validation of the selected biological conditions for.
Figure S1. Alignment of sequences from the 5′-end to the Sm binding site of reported genomic sequences (9-15) for HSUR 1. MicroRNA binding sites are.
KAYAKING EXAMPLE 4 Write and solve a linear system During a kayaking trip, a kayaker travels 12 miles upstream (against the current) and 12 miles downstream.
Linear Prediction Correlation can be used to make predictions – Values on X can be used to predict values on Y – Stronger relationships between X and Y.
Biases in RNA-Seq data. Transcript length bias Two transcripts of length 50 and 100 have the same abundance in a control sample. The expression of both.
Comparative transcriptomics of fungi Group Nicotiana Daan van Vliet, Dou Hu, Joost de Jong, Krista Kokki.
TRANSCRIPTION (DNA → mRNA). Fig. 17-7a-2 Promoter Transcription unit DNA Start point RNA polymerase Initiation RNA transcript 5 5 Unwound.
Supplementary figure 5.
Thursday, March 2, 2017 GOALS: Finish Ghost in your Genes
MLANA/MART1 and SILV/PMEL17/GP100 Are Transcriptionally Regulated by MITF in Melanocytes and Melanoma  Jinyan Du, Arlo J. Miller, Hans R. Widlund, Martin.
Figure 1. Gene expression analysis
Figure 1. Annotation and characterization of genomic target of p63 in mouse keratinocytes (MK) based on ChIP-Seq. (A) Scatterplot representing high degree.
Figure S2 A B Log2 Fold Change (+/- cAMP) Transcriptome (9hr)
Comparison of the Predictive Accuracy of DNA Array-Based Multigene Classifiers across cDNA Arrays and Affymetrix GeneChips  James Stec, Jing Wang, Kevin.
Figure 1. Effect of acute TNF treatment on transcription in human SGBS adipocytes as assessed by RNA-seq and RNAPII ChIP-seq. Following 10 days in vitro.
Supplementary Figure 1 (log fold change relative to untreated control)
5' breakpoint in intron 2 (chr19:1,219,187-1,219,238 shown)
Pick a Gene Assignment 4 Requirements
by Leighton J. Core, Joshua J. Waterfall, and John T. Lis
DNase‐HS sites are main independent determinants of DNA replication timing Simulations based on genome sequence features (GC content, CpG islands), or.
2.6 Draw Scatter Plots and Best-Fitting Lines
GO Annotation from different sources
Sensitivity of RNA‐seq.
Jacek Majewski  The American Journal of Human Genetics 
Simultaneous Isolation of DNA and RNA from the Same Cell Population Obtained by Laser Capture Microdissection for Genome and Transcriptome Profiling 
Volume 10, Issue 7, Pages (July 2017)
Genomic alterations in breast cancer cell line MDA-MB-231.
Volume 18, Issue 9, Pages (February 2017)
Solve Linear Systems by Adding or Subtracting
Volume 44, Issue 1, Pages (October 2011)
Comparison of the Predictive Accuracy of DNA Array-Based Multigene Classifiers across cDNA Arrays and Affymetrix GeneChips  James Stec, Jing Wang, Kevin.
Mapping Whole-Transcriptome Splicing in Mouse Hematopoietic Stem Cells
Supplementary Figure 3. Bisulfite sequencing of FLJ21062 and ONECUT2 CpG islands. The relative positions of the forward (left-most) and reverse (right-most)
Volume 33, Issue 4, Pages e6 (April 2018)
Structure of the 5′ Portion of the Human Plakoglobin Gene
Volume 33, Issue 4, Pages (February 2009)
Downstream antagonism.
Supplemental Figure 3 A B C T-DNA 1 2 RGLG1 2329bp 3 T-DNA 1 2 RGLG2
Protective effect of oestrogen receptor α-PvuII transition against idiopathic male infertility: a case-control study and meta-analysis  Narges Mobasseri,
Volume 23, Issue 1, Pages 9-22 (January 2013)
Volume 14, Issue 7, Pages (February 2016)
Volume 16, Issue 8, Pages (August 2016)
Presence of motifs correlated with decreased expression of downstream genes. Presence of motifs correlated with decreased expression of downstream genes.
Yasunori Aizawa, Qing Xiang, Alan M. Lambowitz, Anna Marie Pyle 
MiR-205 and MiR-375 MicroRNA Assays to Distinguish Squamous Cell Carcinoma from Adenocarcinoma in Lung Cancer Biopsies  Santosh Patnaik, MD, PhD, Reema.
Volume 16, Issue 6, Pages (December 2004)
Genome-wide binding sites of OsMADS1 and the distribution of binding sites in different regions of annotated genes. Genome-wide binding sites of OsMADS1.
A Distinct DNA-Methylation Boundary in the 5′- Upstream Sequence of the FMR1 Promoter Binds Nuclear Proteins and Is Lost in Fragile X Syndrome  Anja Naumann,
Volume 21, Issue 9, Pages (November 2017)
Volume 122, Issue 6, Pages (September 2005)
PKA mediates the primary transcriptional response of cells to glucose.
Graphing with X- and Y-Intercepts
Wook Lew  Journal of Investigative Dermatology 
Effect of rare SVs on cardiac transcription in DCM
Construction of a Rice Glycosyltransferase Phylogenomic Database and Identification of Rice-Diverged Glycosyltransferases  Cao Pei-Jian , Bartley Laura.
Volume 10, Issue 2, Pages (January 2015)
Transcripts enriched and depleted in NB TICs compared with SKPs and other tumor tissues. Transcripts enriched and depleted in NB TICs compared with SKPs.
by Leighton J. Core, Joshua J. Waterfall, and John T. Lis
Mutant TERT promoter displays active histone marks and distinct long-range interactions: A, cell lines that were used in the study with their origin and.
Presentation transcript:

Supplementary Figure 4. Comparisons of MethyLight and gene expression data. PMR values (X-axis) were plotted against log2 gene expression values (Y-axis) obtained from analyses on Affymetrix Human Genome U133 Plus 2.0 Arrays. The equation for a linear fit of the data and correlation coefficient are provided along with the location of the MethyLight reaction relative to the proximal NCBI-designated Reference Sequence (RefSeq) transcript.

HB-266 is 287-bp upstream of start REFSEQ NM_000484

HB-266 is 287-bp upstream of start REFSEQ NM_000484

HB-266 is 287-bp upstream of start REFSEQ NM_000484

HB-249 is 9-bp upstream of start REFSEQ NM_000044

HB-258 is in exon 99-bp downstream of start REFSEQ NM_001709

HB-258 is in exon 99-bp downstream of start REFSEQ NM_001709

HB-363 is 32-bp upstream of start REFSEQ NM_004052

HB-363 is 32-bp upstream of start REFSEQ NM_004052

HB-166 is 93-bp upstream of start REFSEQ NM_001033953

HB-166 is 93-bp upstream of start REFSEQ NM_001033953

HB-146 is 168-bp upstream of start REFSEQ NM_053056

HB-146 is 168-bp upstream of start REFSEQ NM_053056

HB-075 is in exon 132-bp downstream of start REFSEQ NM_001257

HB-075 is in exon 132-bp downstream of start REFSEQ NM_001257

HB-328 is in exon 168-bp downstream of start REFSEQ NM_000076

HB-328 is in exon 168-bp downstream of start REFSEQ NM_000076

HB-329 is in exon 986-bp downstream of start REFSEQ NM_000076

HB-329 is in exon 986-bp downstream of start REFSEQ NM_000076

HB-081 is in exon 306-bp downstream of start REFSEQ NM_058197

HB-081 is in exon 306-bp downstream of start REFSEQ NM_058197

HB-173 is in exon 332-bp downstream of start REFSEQ NM_004936

HB-195 is in exon 4-bp downstream of start REFSEQ NM_001804

HB-480 is 247-bp upstream of start REFSEQ NM_001806

HB-480 is 247-bp upstream of start REFSEQ NM_001806

HB-310 is in exon 57-bp downstream of start REFSEQ NM_001307

HB-193 is in exon 342-bp downstream of start REFSEQ NM_000089

HB-197 is in exon 37-bp downstream of start REFSEQ NM_004378

HB-054 is 97-bp upstream of start REFSEQ NM_001338

HB-054 is 97-bp upstream of start REFSEQ NM_001338

HB-054 is 97-bp upstream of start REFSEQ NM_001338

HB-223 is in intron 512-bp downstream of start REFSEQ NM_000785

HB-218 is in exon 266-bp downstream of start REFSEQ NM_006094

HB-218 is in exon 266-bp downstream of start REFSEQ NM_006094

HB-048 is in exon 385-bp downstream of start REFSEQ NM_013238

HB-048 is in exon 385-bp downstream of start REFSEQ NM_013238

HB-252 is 463-bp upstream of start REFSEQ NM_000794

HB-164 is in exon 143-bp downstream of start REFSEQ NM_000125

HB-164 is in exon 143-bp downstream of start REFSEQ NM_000125

HB-164 is in exon 143-bp downstream of start REFSEQ NM_000125

HB-164 is in exon 143-bp downstream of start REFSEQ NM_000125

HB-442 is 21-bp upstream of start REFSEQ NM_001039706

HB-254 is 423-bp upstream of start REFSEQ NM_000807

HB-255 is 847-bp upstream of start REFSEQ NM_000817

HB-255 is 847-bp upstream of start REFSEQ NM_000817

HB-327 is 371-bp upstream of start REFSEQ NM_001002295

HB-323 is in intron 394-bp downstream of start REFSEQ NM_002052

HB-323 is in intron 394-bp downstream of start REFSEQ NM_002052

HB-323 is in intron 394-bp downstream of start REFSEQ NM_002052

HB-324 is in exon 3625-bp downstream of start REFSEQ NM_002052

HB-324 is in exon 3625-bp downstream of start REFSEQ NM_002052

HB-324 is in exon 3625-bp downstream of start REFSEQ NM_002052

HB-357 is 93-bp upstream of start REFSEQ NM_004864

HB-222 is in exon 4932-bp downstream of start REFSEQ NM_000514

HB-426 is 9-bp upstream of start REFSEQ NM_018960

HB-250 is 863-bp upstream of start REFSEQ NM_000834

HB-250 is 863-bp upstream of start REFSEQ NM_000834

HB-168 is in intron 1648-bp downstream of start REFSEQ NM_006497

HB-168 is in intron 1648-bp downstream of start REFSEQ NM_006497

HB-440 is in exon 536-bp downstream of start REFSEQ NM_006897

HB-069 is in exon 37-bp downstream of start REFSEQ NM_014333

HB-069 is in exon 37-bp downstream of start REFSEQ NM_014333

HB-069 is in exon 37-bp downstream of start REFSEQ NM_014333

HB-069 is in exon 37-bp downstream of start REFSEQ NM_014333

HB-069 is in exon 37-bp downstream of start REFSEQ NM_014333

HB-069 is in exon 37-bp downstream of start REFSEQ NM_014333

HB-175 is 10-bp upstream of start REFSEQ NM_004795

HB-481 is in intron 2041-bp downstream of start REFSEQ NM_020805

HB-219 is 219-bp upstream of start REFSEQ NM_000527

HB-219 is 219-bp upstream of start REFSEQ NM_000527

HB-219 is 219-bp upstream of start REFSEQ NM_000527

HB-070 is in exon 2461-bp downstream of start REFSEQ NM_019839

HB-159 is in intron 327-bp downstream of start REFSEQ NM_002412

HB-160 is in exon 48-bp downstream of start REFSEQ NM_002412

HB-204 is 82-bp upstream of start REFSEQ NM_005950

HB-207 is in exon 177-bp downstream of start REFSEQ NM_005954

HB-058 is in exon 3093-bp downstream of start REFSEQ NM_005957

HB-058 is in exon 3093-bp downstream of start REFSEQ NM_005957

HB-058 is in exon 3093-bp downstream of start REFSEQ NM_005957

HB-058 is in exon 3093-bp downstream of start REFSEQ NM_005957

HB-259 is in exon 35-bp downstream of start REFSEQ NM_002500

HB-260 is in exon 58-bp downstream of start REFSEQ NM_006160

HB-320 is 40-bp upstream of start REFSEQ NM_033120

HB-485 is in exon 159-bp downstream of start REFSEQ NM_014697

HB-485 is in exon 159-bp downstream of start REFSEQ NM_014697

HB-242 is 447-bp upstream of start REFSEQ NM_004852 *HB-242average HB-242 is 447-bp upstream of start REFSEQ NM_004852

HB-242 is 447-bp upstream of start REFSEQ NM_004852

HB-242 is 447-bp upstream of start REFSEQ NM_004852

HB-243 is in intron 2387-bp downstream of start REFSEQ NM_004852 *HB-243average HB-243 is in intron 2387-bp downstream of start REFSEQ NM_004852

HB-243 is in intron 2387-bp downstream of start REFSEQ NM_004852

HB-243 is in intron 2387-bp downstream of start REFSEQ NM_004852

HB-446 is in intron 2387-bp downstream of start REFSEQ NM_004852

HB-483 is in intron 26138-bp downstream of start REFSEQ NM_198040

HB-483 is in intron 26138-bp downstream of start REFSEQ NM_198040

HB-482 is 523-bp upstream of start REFSEQ NM_178013

HB-149 is in exon 2939-bp downstream of start REFSEQ NM_000926

HB-149 is in exon 2939-bp downstream of start REFSEQ NM_000926

HB-169 is in exon 903-bp downstream of start REFSEQ NM_000926

HB-169 is in exon 903-bp downstream of start REFSEQ NM_000926

HB-060 is 8-bp upstream of start REFSEQ NM_138712

HB-176 is in exon 77-bp downstream of start REFSEQ NM_000965

HB-176 is in exon 77-bp downstream of start REFSEQ NM_000965

HB-176 is in exon 77-bp downstream of start REFSEQ NM_000965

HB-176 is in exon 77-bp downstream of start REFSEQ NM_000965

HB-322 is in exon 300-bp downstream of start REFSEQ NM_206963

HB-322 is in exon 300-bp downstream of start REFSEQ NM_206963

HB-044 is in exon 216-bp downstream of start REFSEQ NM_007182

HB-185 is in exon 43-bp downstream of start REFSEQ NM_002899

HB-185 is in exon 43-bp downstream of start REFSEQ NM_002899

HB-085 is in exon 274-bp downstream of start REFSEQ NM_015278

HB-085 is in exon 274-bp downstream of start REFSEQ NM_015278

HB-085 is in exon 274-bp downstream of start REFSEQ NM_015278

HB-085 is in exon 274-bp downstream of start REFSEQ NM_015278

HB-085 is in exon 274-bp downstream of start REFSEQ NM_015278

HB-387 is 269-bp upstream of start REFSEQ NM_002970

HB-387 is 269-bp upstream of start REFSEQ NM_002970

HB-194 is in intron 137-bp downstream of start REFSEQ NM_052863

HB-184 is 299-bp upstream of start REFSEQ NM_021115

HB-184 is 299-bp upstream of start REFSEQ NM_021115

HB-315 is 40415-bp upstream of start REFSEQ NM_005905

HB-064 is in exon 66-bp downstream of start REFSEQ NM_001018003

HB-215 is 12659-bp downstream of end REFSEQ NM_001032280

HB-215 is 12659-bp downstream of end REFSEQ NM_001032280

HB-215 is 12659-bp downstream of end REFSEQ NM_001032280

HB-145 is 261-bp upstream of start REFSEQ NM_003225

HB-244 is in intron 3364-bp downstream of start REFSEQ NM_003225

HB-361 is in exon 68-bp downstream of start REFSEQ NM_006528

HB-247 is 636-bp upstream of start REFSEQ NM_003246

HB-247 is 636-bp upstream of start REFSEQ NM_003246

HB-247 is 636-bp upstream of start REFSEQ NM_003246

HB-443 is in intron 368-bp downstream of start REFSEQ NM_006034

HB-443 is in intron 368-bp downstream of start REFSEQ NM_006034

HB-047 is in exon 744-bp downstream of start REFSEQ NM_000474

HB-364 is in intron 86851-bp downstream of start REFSEQ NM_199427

HB-431 is 72-bp upstream of start REFSEQ NM_198480