Phosphatase.

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Presentation transcript:

Phosphatase

‘Experiments on product inhibition with phosphatase’ Higher Biology Metabolism and Survival: 1. Metabolic pathways and their control - Control of metabolic pathways ‘Experiments on product inhibition with phosphatase’

‘Experiments on product inhibition with phosphatase’ Higher Human Biology Human Cells: 6. Metabolic pathways - Control of metabolic pathways ‘Experiments on product inhibition with phosphatase’

Phosphatase Phosphatase enzymes from plants: a versatile resource for post-16 courses Meatyard, B. (1999), J. Biol. Educ., 33(2), 109-112.

The Assay

PP = phenolphthalein (product) PPP = phenolphthalein phosphate (substrate)

Preparing the enzyme extract (Part A) Running the assay (Part B)

Creating the extract

Germination

Maceration Distilled water

Loading centrifuge tube

Centrifugation

Retain supernatant

Running the Assay

Running the assay

Investigations Effect of temperature Effect of pH Phosphatase levels from different species Phosphatase levels in different locations Effect of phosphate concentration

Results

Results